FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PRDX3 with M25079 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Mouse anti-Human PRDX3 Monoclonal Antibody | anti-PRDX3 antibody
PRDX3 Mouse mAb
Gene Names
PRDX3; AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
PRDX3, Antibody; PRDX3 Mouse mAb; anti-oxidant protein 1; antioxidant protein; Antioxidant protein 1; AOP-1; HBC189; mitochondrial thioredoxin dependent peroxide reductase; mitochondrial Trx dependent peroxide reductase; Perioredoxin-3; Peroxiredoxin III; Peroxiredoxin-3; Protein MER5; Protein MER5 homolog; PRX III; PRX-3; Prx-III; Prx3; Thioredoxin-dependent peroxide reductase, mitochondrial; AOP1; AW822249; D0Tohi1; Ef2l; MER5; PRDX3; PRO1748; prx-III; SP-22; SP22; TDXM; anti-PRDX3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Sequence Length
198
Applicable Applications for anti-PRDX3 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human PRDX3
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PRDX3 with M25079 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PRDX3 with M25079 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation)
(PRDX3 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25079 of 10 ug. Western blot was performed from the immunopre-cipitate using M25079 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 27 KDaObserved band size : 27 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
IP (Immunoprecipitation)
(PRDX3 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25079 of 10 ug. Western blot was performed from the immunopre-cipitate using M25079 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 27 KDaObserved band size : 27 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot)
WB (Western Blot)
Related Product Information for anti-PRDX3 antibody
This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22.
Product Categories/Family for anti-PRDX3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 27kDa
NCBI Official Full Name
thioredoxin-dependent peroxide reductase, mitochondrial isoform c
NCBI Official Synonym Full Names
peroxiredoxin 3
NCBI Official Symbol
PRDX3
NCBI Official Synonym Symbols
AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
NCBI Protein Information
thioredoxin-dependent peroxide reductase, mitochondrial
UniProt Protein Name
Thioredoxin-dependent peroxide reductase, mitochondrial
UniProt Gene Name
PRDX3
UniProt Synonym Gene Names
AOP1; AOP-1; Prx-III
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Product Notes
The PRDX3 prdx3 (Catalog #AAA62306) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRDX3 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRDX3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PRDX3 prdx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDX3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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