WB (Western Blot)
(Detection of mouse PTPN12 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
PTPN12 recombinant antibody
Rabbit anti-PTPN12 Recombinant Monoclonal Antibody [BL-5-2F8]
IP: 20 ul/mg lysate
IHC: 1:20-1:200. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:20-1:200. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse PTPN12 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse PTPN12 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PTPN12 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 [SK], and HCT 116 prepared using NETN lysis buffer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human PTPN12 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 [SK], and HCT 116 prepared using NETN lysis buffer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PTPN12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used for IP at 20 ul per reaction. PTPN12 was also immunoprecipitated by rabbit anti-PTPN12 antibody For blotting immunoprecipitated PTPN12, AAA23788 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PTPN12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used for IP at 20 ul per reaction. PTPN12 was also immunoprecipitated by rabbit anti-PTPN12 antibody For blotting immunoprecipitated PTPN12, AAA23788 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human PTPN12 by immunohistochemistry. Sample: FFPE section of non-small cell lung cancer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human PTPN12 by immunohistochemistry. Sample: FFPE section of non-small cell lung cancer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human PTPN12 by immunohistochemistry. Sample: FFPE section of mammary gland. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human PTPN12 by immunohistochemistry. Sample: FFPE section of mammary gland. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human PTPN12 by immunocytochemistry. Sample: FFPE section of SW620 cells. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:40. Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human PTPN12 by immunocytochemistry. Sample: FFPE section of SW620 cells. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (AAA23788 lot 1) used at 1:40. Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
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Product Notes
The PTPN12 ptpn12 (Catalog #AAA23788) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PTPN12 Recombinant Monoclonal Antibody [BL-5-2F8] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PTPN12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:20-1:200. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC: 1:20-1:200. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Researchers should empirically determine the suitability of the PTPN12 ptpn12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPN12, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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