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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with PU.1/Spi1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human PU.1/Spi1 Monoclonal Antibody | anti-PU.1/Spi1 antibody
PU.1/Spi1 Antibody
Gene Names
SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
PU.1/Spi1, Antibody; PU.1/Spi1 Antibody; transcription factor spi1 antibody; 31 kDa Transforming Protein antibody; 31 kDa-transforming protein antibody; cb1086 antibody; Hematopoietic transcription factor PU.1 antibody; OF antibody; oncogene spi1 antibody; PU.1 antibody; SFFV virus-induced murine erythroleukemia oncogene; mouse; homolog of antibody; SFPI1 antibody; si:by184l24.2 antibody; SPI 1 antibody; SPI 1 proto oncogene antibody; SPI A antibody; Spi1 antibody; SPI1_HUMAN antibody; Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody; Spleen focus forming virus proviral integration oncogene spi1 antibody; Transcription factor PU.1 antibody; anti-PU.1/Spi1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JB72-35
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PU.1/Spi1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with PU.1/Spi1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with PU.1/Spi1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution.)
Related Product Information for anti-PU.1/Spi1 antibody
The Ets transcription factor family (Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T-cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T-lineage commitment and then is dramatically absent following the beta-selection checkpoint.
Product Categories/Family for anti-PU.1/Spi1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,083 Da
NCBI Official Full Name
transcription factor PU.1 isoform 1
NCBI Official Synonym Full Names
spleen focus forming virus (SFFV) proviral integration oncogene spi1
NCBI Official Symbol
SPI1
NCBI Official Synonym Symbols
OF; PU.1; SFPI1; SPI-1; SPI-A
NCBI Protein Information
transcription factor PU.1; SPI-1 proto-oncogene; 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1
UniProt Protein Name
Transcription factor PU.1
UniProt Gene Name
SPI1
UniProt Entry Name
SPI1_HUMAN
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Product Notes
The PU.1/Spi1 spi1 (Catalog #AAA312627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PU.1/Spi1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PU.1/Spi1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PU.1/Spi1 spi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PU.1/Spi1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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