WB (Western Blot)
(SPI1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The SPI1 antibody detected the SPI1 protein (arrow).)
Rabbit anti-Human SPI1 / PU.1 Polyclonal Antibody | anti-SPI1 / PU.1 antibody
Anti-SPI1 / PU.1 Antibody (aa216-246) IHC-plus
Gene Names
SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity purified
Synonyms
SPI1 / PU.1, Antibody; Anti-SPI1 / PU.1 Antibody (aa216-246) IHC-plus; Rabbit Polyclonal to Human SPI1 / PU.1; Human SPI1 / PU.1; 31 kDa-transforming protein; 31 kDa transforming protein; SFPI1; SPI-1; SPI-1 proto-oncogene; SPI-A; Transcription factor PU.1; anti-SPI1 / PU.1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human PU.1
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.09% sodium azide
Sequence Length
270
Applicable Applications for anti-SPI1 / PU.1 antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
SPI1 / PU.1 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human SPI1.
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human SPI1.
Immunogen Type
Synthetic peptide
Antigen Modification
aa216-246
Target Species
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
For long term storage store at -20 degree C.
WB (Western Blot)
(SPI1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The SPI1 antibody detected the SPI1 protein (arrow).)
WB (Western Blot)
(SPI1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The SPI1 antibody detected the SPI1 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody. NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SPI1 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody. NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SPI1 immunoreactivity is localized to Nucleus significantly.)
IHC (Immunohistochemistry)
(Anti-SPI1 / PU.1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
IHC (Immunohistochemistry)
(Anti-SPI1 / PU.1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Related Product Information for anti-SPI1 / PU.1 antibody
SPI1 Antibody, 31 kDa-transforming protein Antibody, 31 kDa transforming protein Antibody, PU.1 Antibody, SFPI1 Antibody, SPI-1 Antibody, SPI-1 proto-oncogene Antibody, SPI-A Antibody, Transcription factor PU.1 Antibody Description: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,211 Da
NCBI Official Full Name
transcription factor PU.1 isoform 2
NCBI Official Synonym Full Names
Spi-1 proto-oncogene
NCBI Official Symbol
SPI1
NCBI Official Synonym Symbols
OF; PU.1; SFPI1; SPI-1; SPI-A
NCBI Protein Information
transcription factor PU.1
UniProt Protein Name
Transcription factor PU.1
UniProt Gene Name
SPI1
UniProt Entry Name
SPI1_HUMAN
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Product Notes
The SPI1 / PU.1 spi1 (Catalog #AAA162160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SPI1 / PU.1 Antibody (aa216-246) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPI1 / PU.1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SPI1 / PU.1 spi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPI1 / PU.1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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