Mouse anti-Human RIG I /DDx58 Monoclonal Antibody | anti-RIG I/DDx58 antibody

Monoclonal Antibody to RIG-I (Clone: ABM4H29)

Cat. Num. AAA14869

• Gene Names: DDX58; RIGI; RIG-I; RLR-1; SGMRT2
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Protein G chromatography
• Synonyms: RIG I /DDx58, Antibody; Monoclonal Antibody to RIG-I (Clone: ABM4H29); anti-RIG I/DDx58 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1, Kappa
• Clone Number: ABM4H29
• Purity/Purification: Protein G chromatography
• Form/Format: Purified; 25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
• Sequence Length: 925

• Applicable Applications for anti-RIG I/DDx58 antibody: Immunohistochemistry (IHC), Flow Cytometry(FACS), Western Blot(WB)
• Application Notes: Western blot analysis: 2-4ug/ml; Immunohistochemical analysis: 15ug/ml; Flow Cytometric analysis: 0.5-1ug/10^6 cells
• Immunogen: A partial length recombinant human RIG-1 protein (amino acids 1-220) was used as the immunogen for this antibody.
• Preparation and Storage: Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

FCM (Flow Cytometry)

(Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM4H29). Green represent isotype control and red represent Anti-RIG I antibody (AAA14869). Goat anti-mouse PE conjugate was used as secondary.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of RIG1/DDx58 in human Spleen Tissue using RIG1/DDx58 antibody (Clone: ABM4H29) at 15 ug/ml.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of RIG1/DDx58 in human Colon Tissue using RIG1/DDx58 antibody (Clone: ABM4H29) at 15 ug/ml.)

WB (Western Blot)

(Expression analysis of RIG1/DDx58. Anti-RIG1/DDx58 antibody (Clone: ABM4H29) was tested at 2 ug/ml on human kidney lysate.)

Related Product Information for anti-RIG I/DDx58 antibody

RIG-I (retinoic-acid-inducible gene I), also known as DDX58 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 58) is a 925-residue cytoplasmic viral RNA receptor, critically involved in the activation of the innate immune response to RNA virus infection. It is a member of the RIG-I-like receptor (RLR) family and is an essential intracellular sensor for several 5?-triphosphorylated RNA viruses. RIG-I elicits its antiviral interferon (IFN) responses by recognizing viral double-stranded RNAs (dsRNAs). Structurally it comprises a helicase domain, a C-terminal domain, and N-terminal CARDs (caspase activation recruitment domains) involved in activating MAVS (mitochondrial antiviral signaling protein). Upon binding of 5?-triphosphorylated RNA, RIG-I undergoes conformational changes and post-translational modifications that allow multimerization and interaction with the mitochondrial antiviral signaling protein (MAVS).

Product Categories/Family for anti-RIG I/DDx58 antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 27881482
• NCBI GeneID: 23586
• NCBI Accession #: NP_055129.2
• NCBI GenBank Nucleotide #: NM_014314.3
• UniProt Accession #: O95786; Q5HYE1; Q5VYT1; Q9NT04; A2RU81
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX58
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• NCBI Official Symbol: DDX58
• NCBI Official Synonym Symbols: RIGI; RIG-I; RLR-1; SGMRT2
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX58
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX58
• UniProt Gene Name: DDX58
• UniProt Synonym Gene Names: RLR-1; RIG-1; RIG-I
• UniProt Entry Name: DDX58_HUMAN