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WB (Western Blot)
(Detection of human S100A9 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, HL-60, HEK293T, MUTZ-3, and THP-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH antibody .)
S100A9 recombinant antibody
Rabbit anti-S100A9 Recombinant Monoclonal Antibody [BLR205K]
Gene Names
S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
S100A9, Recombinant Antibody; Rabbit anti-S100A9 Recombinant Monoclonal Antibody [BLR205K]; MAC387; MIF; migration inhibitory factor-related protein 14; MRP14; MRP-14; NIF; P14; protein S100-A9; CGLB; S100 calcium-binding protein A9; CAGB; leukocyte L1 complex heavy chain; calgranulin B; Calgranulin-B; calprotectin L1H subunit; CFAG; LIAG; L1AG; 60B8AG; Protein S100-A9; S100 calcium binding protein A9; S100A9 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR205K
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
25ug/ml (varies by lot)
Applicable Applications for S100A9 recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 64 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human S100A9 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, HL-60, HEK293T, MUTZ-3, and THP-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH antibody .)
WB (Western Blot)
(Detection of human S100A9 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, HL-60, HEK293T, MUTZ-3, and THP-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH antibody .)
IHC (Immunohistochemistry)
(Detection of human S100A9 by immunohistochemistry. Sample: FFPE section of spleen. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human S100A9 by immunohistochemistry. Sample: FFPE section of spleen. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemisry)
(Detection of human S100A9 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemisry)
(Detection of human S100A9 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human S100A9 by immunocytochemistry. Sample: FFPE section of MUTZ-3 cells. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human S100A9 by immunocytochemistry. Sample: FFPE section of MUTZ-3 cells. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human S100A9 by immunocytochemistry. Sample: FFPE section of NK-92 cells. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human S100A9 by immunocytochemistry. Sample: FFPE section of NK-92 cells. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Related Product Information for S100A9 recombinant antibody
S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. [taken from NCBI Entrez Gene (Gene ID: 6280)].
Product Categories/Family for S100A9 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,242 Da
NCBI Official Full Name
protein S100-A9
NCBI Official Synonym Full Names
S100 calcium binding protein A9
NCBI Official Symbol
S100A9
NCBI Official Synonym Symbols
MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
NCBI Protein Information
protein S100-A9; MRP-14; S100 calcium-binding protein A9 (calgranulin B); calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14
UniProt Protein Name
Protein S100-A9
UniProt Gene Name
S100A9
UniProt Synonym Gene Names
CAGB; CFAG; MRP14; MRP-14; p14
UniProt Entry Name
S10A9_HUMAN
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Product Notes
The S100A9 s100a9 (Catalog #AAA213638) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-S100A9 Recombinant Monoclonal Antibody [BLR205K] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's S100A9 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the S100A9 s100a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A9, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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