SARS-CoV-2 (2019-nCoV) Spike S2 Monoclonal Antibody | anti-SARS-CoV-2 antibody

SARS-CoV-2 (2019-nCoV) Spike S2 Antibody, Chimeric MAb

Cat. Num. AAA257787

• Reactivity: SARS-CoV-2
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A
• Synonyms: SARS-CoV-2 (2019-nCoV) Spike S2, Antibody; SARS-CoV-2 (2019-nCoV) Spike S2 Antibody, Chimeric MAb; anti-SARS-CoV-2 antibody

• Host: Chimeric Mouse/Human
• Reactivity: SARS-CoV-2
• Clonality: Monoclonal
• Isotype: Mouse (variable region)/Human (kappa/IgG1 constant) chimeric antibody
• Clone Number: D001
• Specificity: SARS-CoV-2 (2019-nCoV) Spike S2 Protein
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-SARS-CoV-2 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Recombinant SARS-CoV-2/2019-nCoV Spike S2 ECD Protein
• Conjugation: Unconjugated
• Preparation: It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV-2 Spike S2 ECD Protein. The antibody was produced using recombinant antibody technology.
• Cross-reactivity: Has cross-reactivity in ELISA with; SARS-CoV-2 Spike S1+S2 ECD Protein ; SARS-CoV Spike S1+S2 ECD Protein ; MERS-CoV Spike S1+S2 ECD Protein ; No cross-reactivity in ELISA with; MERS-CoV Spike Spike S2 ; SARS-CoV-2 (2019-nCoV) Spike S1 ; SARS-CoV-2 (2019-nCoV) Spike RBD ; HCoV-OC43-Spike S1+S2 Protein ; HCoV-229E-Spike S1+S2 Protein ; HCoV-HKU1 (isolate N5)Spike S1+S2 Protein ; HCoV-NL63-Spike S1+S2 Protein
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IF (Immunofluorescence)

(Immunofluorescence analysis of SARS-COV-2 Spike overexpressed HEK293 Cells were stained with purified anti-SARS-CoV-2 Spike Chimera Mab,then a FITC-conjugated second step antibody.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of SARS-COV-2 Spike overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Spike Chimeric MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

References

Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.