ELISA
(Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1ug/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.)
SARS-CoV-2 (COVID-19) Spike S2 Recombinant Antibody | SARS-CoV-2 recombinant antibody
SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1B8]
Reactivity
Virus
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
Synonyms
SARS-CoV-2 (COVID-19) Spike S2, Recombinant Antibody; SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1B8]; SARS-CoV-2 (COVID-19) Spike S2 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Surface Glycoprotein; Spike protein; surface glycoprotein; SARS-CoV-2 recombinant antibody
Host
Llama
Reactivity
Virus
Clonality
Monoclonal
Clone Number
P1B8
Purity/Purification
SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
Form/Format
Liquid; SARS-CoV-2 (COVID-19) Spike S2 Antibody is supplied in PBS.
Applicable Applications for SARS-CoV-2 recombinant antibody
ELISA
Immunogen
SARS-CoV-2 Spike S2 protein containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Ser 686 - Pro 1213.
Research Area
Infectious Disease, COVID-19
NCBI Organism
SARS-CoV-2
Preparation and Storage
SARS-CoV-2 (COVID-19) Spike S2 Antibody should be stored in working aliquots at -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ELISA
(Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1ug/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.)
ELISA
(Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1ug/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.)
ELISA
(Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, AAA40985. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA40985) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.)
ELISA
(Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, AAA40985. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA40985) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.)
Related Product Information for SARS-CoV-2 recombinant antibody
As SARS-CoV-2 Spike S2 protein is more conserved among coronaviruses than Spike S1 protein, antibodies against spike S2 protein may react with more Coronaviruses, serving as a pan-coronvirus antibody. The functional characteristics analysis revealed that P1B8 has excellent binding activity to SARS-CoV-2 variants (delta and omicron), but weaker binding activity to SARS-CoV-1. Biotinylated P1B8 can still bind spike trimer of SARS-CoV-2. In addition, P1B8 and another three spike S2 sdAbs (P1G5, P1A9, and P1A6) belong to different families based on their sequence alignment, and epitope mapping experiment indicated that they may bind to different epitopes with different affinity to the different strains in the binding ELISA. Our data showed that these spike S2 sdAbs bind spike trimer of both SARS-CoV-2 and SARS-CoV-1, suggesting that these spike S2 sdAbs have potentials as a novel and efficient tool suitable for pan-coronavirus studies as well as for identification of various coronaviruses and their variants from clinical samples.
Product Categories/Family for SARS-CoV-2 recombinant antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SARS-CoV-2 (Catalog #AAA40985) is a Recombinant Antibody produced from Llama and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1B8] reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's SARS-CoV-2 (COVID-19) Spike S2 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the SARS-CoV-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARS-CoV-2 (COVID-19) Spike S2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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