Application Data
(Figure 5 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529),Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, AAA40969, 2ug/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant Spike antibody (AAA40969) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and all other variants. )
Rabbit anti-Virus SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Polyclonal Antibody | anti-SARS-CoV-2 antibody
SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Antibody (Omicron)
The immunogen is located within 480-530 amino acids of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.
Application Data
(Figure 5 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529),Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, AAA40969, 2ug/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant Spike antibody (AAA40969) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and all other variants. )
Application Data
(Figure 5 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529),Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, AAA40969, 2ug/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant Spike antibody (AAA40969) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and all other variants. )
Application Data
(Figure 4 SARS-Cov-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike RBD Protein in an ELISA Coating Antigen: SARS-CoV-2 spike RBD proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1ug/mL, incubated at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, AAA40969, 1ug/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.)
Application Data
(Figure 4 SARS-Cov-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike RBD Protein in an ELISA Coating Antigen: SARS-CoV-2 spike RBD proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1ug/mL, incubated at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, AAA40969, 1ug/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.)
Application Data
(Figure 3 SARS-Cov-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1ug/mL, incubated at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, AAA40969, 1ug/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.)
Application Data
(Figure 3 SARS-Cov-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1ug/mL, incubated at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, AAA40969, 1ug/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.)
Application Data
(Figure 2 Neutralization Assay of Omicron Variant Pseudovirus by SARS-CoV-2 Omicron Variant Spike RBD Antibody Neutralization antibodies: Anti-SARS-CoV-2 Spike RBD antibodies, AAA40969, dilution: 0.3 - 30,000 ng/mL.Pseudovirus: SARS-CoV-2 Omicron Spike Pseudovirus, 95-201.Infected Cells: ACE2-overexpressing 293T cellsDiluted antibody was incubated with Omicron pseudovirus at 37 degree C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 degree C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader.Percent inhibition is calculated based on the RLU value.)
Application Data
(Figure 2 Neutralization Assay of Omicron Variant Pseudovirus by SARS-CoV-2 Omicron Variant Spike RBD Antibody Neutralization antibodies: Anti-SARS-CoV-2 Spike RBD antibodies, AAA40969, dilution: 0.3 - 30,000 ng/mL.Pseudovirus: SARS-CoV-2 Omicron Spike Pseudovirus, 95-201.Infected Cells: ACE2-overexpressing 293T cellsDiluted antibody was incubated with Omicron pseudovirus at 37 degree C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 degree C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader.Percent inhibition is calculated based on the RLU value.)
IF (Immunofluorescence)
(Figure 1 Immunofluorescence Validation of Spike RBD in the Nasal Swab Sample of Omicron Variant COVID-19 Patient. Immunofluorescent analysis of 4% paraformaldehyde-fixed patient nasal swab sample labeling spike RBD with AAA40969 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 1 Immunofluorescence Validation of Spike RBD in the Nasal Swab Sample of Omicron Variant COVID-19 Patient. Immunofluorescent analysis of 4% paraformaldehyde-fixed patient nasal swab sample labeling spike RBD with AAA40969 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Zhang et al. PNAS of U.S.A. 2021, 118(47):e2109905118.
Cameroni et al. Nature 2021.
Rossler et al. NEJM 2022.
Muik et al. Science 2022.
NCBI and Uniprot Product Information
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Product Notes
The SARS-CoV-2 (Catalog #AAA40969) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Antibody (Omicron) reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SARS-CoV-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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