Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using SDHB Mouse Monoclonal Antibody (SDHB/2382). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
Mouse anti-Human SDHB (Succinate Dehydrogenase B) Monoclonal Antibody | anti-SDHB antibody
SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker)
Gene Names
SDHB; IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
Reactivity
Human
Applications
Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
SDHB (Succinate Dehydrogenase B), Antibody; SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker); CWS2; DHSB; Iron-sulfur subunit of complex II; PGL4; SDH2; SDHB; SDHIP; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit; mitochondrial; Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein; anti-SDHB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
SDHB/2382
Specificity
Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function.Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation.Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
280
Applicable Applications for anti-SDHB antibody
WB (Western Blot)
Positive Control
Jurkat or HepG2 cells. Human kidney or liver.
Immunogen
Recombinant fragment (around aa 165-273) of human SDHB protein (exact sequence is proprietary)
Cellular Localization
Cytoplasmic (Mitochondria)
Hu-chromosome Location
1p36.13
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using SDHB Mouse Monoclonal Antibody (SDHB/2382). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using SDHB Mouse Monoclonal Antibody (SDHB/2382). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis of Purified SDHB Mouse Monoclonal Antibody (SDHB/2382). Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis of Purified SDHB Mouse Monoclonal Antibody (SDHB/2382). Confirmation of Integrity and Purity of Antibody.)
References
Allibhai, Z., et al. 2004. Malignant pheochromocytoma associated with germline mutation of the SDHB gene. J. Urol. 172: 1409-1410.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
32kDa
NCBI Official Full Name
Succinate dehydrogenase
NCBI Official Synonym Full Names
succinate dehydrogenase complex iron sulfur subunit B
NCBI Official Symbol
SDHB
NCBI Official Synonym Symbols
IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
NCBI Protein Information
succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
UniProt Protein Name
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
UniProt Gene Name
SDHB
UniProt Synonym Gene Names
SDH; SDH1; Ip
UniProt Entry Name
SDHB_HUMAN
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Product Notes
The SDHB sdhb (Catalog #AAA215191) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SDHB (Succinate Dehydrogenase B) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SDHB sdhb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDHB (Succinate Dehydrogenase B), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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