FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing 293T whole cell lysate transfected with SEMA4D stained with AAA279010 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse anti-Human SEMA4D Monoclonal Antibody | anti-SEMA4D antibody
Mouse anti-Homo sapiens (Human) SEMA4D Monoclonal antibody
Gene Names
SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
SEMA4D, Antibody; Mouse anti-Homo sapiens (Human) SEMA4D Monoclonal antibody; SEMA4D Monoclonal Antibody; Semaphorin-4D (A8)(BB18)(GR3)(CD antigen CD100); SEMA4D; C9orf164 CD100 SEMAJ; anti-SEMA4D antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
10E12E10
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for anti-SEMA4D antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human SEMA4D protein(22-734aa)
Conjugation
Non-conjugated
Research Area
Signal Transduction
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing 293T whole cell lysate transfected with SEMA4D stained with AAA279010 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing 293T whole cell lysate transfected with SEMA4D stained with AAA279010 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in: 293T whole cell lysate transfected with SEMA4D; Recombinant proteins with SEMA4DAll lanes: SEMA4D antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 130, 150 KDaObserved band size: 130, 150 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: 293T whole cell lysate transfected with SEMA4D; Recombinant proteins with SEMA4DAll lanes: SEMA4D antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 130, 150 KDaObserved band size: 130, 150 KDaExposure time?5min)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,150 Da
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
UniProt Protein Name
Semaphorin-4D
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN
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Product Notes
The SEMA4D sema4d (Catalog #AAA279010) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Homo sapiens (Human) SEMA4D Monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA4D can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SEMA4D sema4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA4D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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