FCM/FACS (Flow Cytometry)
(Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
SEMA4D recombinant antibody
SEMA4D Recombinant Monoclonal Antibody
Gene Names
SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Affinity purified
Synonyms
SEMA4D, Recombinant Antibody; SEMA4D Recombinant Monoclonal Antibody; Semaphorin-4D (A8)(BB18)(GR3)(CD antigen CD100); SEMA4D; C9orf164 CD100 SEMAJ; SEMA4D recombinant antibody
Host
HEK293F Cell
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
hIgG4(S228P)
Clone Number
5D12
Purity/Purification
Affinity purified
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for SEMA4D recombinant antibody
FCM/FACS (Flow Cytometry), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human SEMA4D protein
Conjugation
Non-conjugated
Research Area
Immunology
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
FCM/FACS (Flow Cytometry)
(Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
ELISA
(The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D at 2 ug/mL can bind Anti- SEMA4D recombinant antibody, the EC50 is 60.38-152.6 ng/mL.)
ELISA
(The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D at 2 ug/mL can bind Anti- SEMA4D recombinant antibody, the EC50 is 60.38-152.6 ng/mL.)
ELISA
(The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Anti-SEMA4D recombinant antibody at 2 ug/mL can bind Mouse Sema4d , the EC50 is 5.829-16.86 ng/mL.)
ELISA
(The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Anti-SEMA4D recombinant antibody at 2 ug/mL can bind Mouse Sema4d , the EC50 is 5.829-16.86 ng/mL.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,150 Da
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
UniProt Protein Name
Semaphorin-4D
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN
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Product Notes
The SEMA4D sema4d (Catalog #AAA279020) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The SEMA4D Recombinant Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA4D can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the SEMA4D sema4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA4D, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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