WB (Western Blot)
(Blots of HeLa cell crude extract stained with SF3B4 antibody. SF3B4 runs with an apparent SDS-PAGE molecular weight 49kDa.)
Mouse SF3B4 Monoclonal Antibody | anti-SF3B4 antibody
Mouse Monoclonal [clone 3A1] (IgG2b,k) to Human SF3B4
Gene Names
SF3B4; AFD1; Hsh49; SAP49; SF3b49
Reactivity
Bovine, Human, Mouse, Pig, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Purified
Synonyms
SF3B4, Antibody; Mouse Monoclonal [clone 3A1] (IgG2b,k) to Human SF3B4; Anti-SF3B4 Antibody (clone 3A1) IHC-plus; SF3B4; AFD1; Hsh49; SAP 49; Spliceosomal protein; SF3b50; SAP49; SF3b49; Splicing factor 3B subunit 4; Human SF3B4; anti-SF3B4 antibody
Host
Mouse
Reactivity
Bovine, Human, Mouse, Pig, Rat
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3A1
Specificity
Clone 3A1 is known to react with SF3B4 from human, cow, pig, mouse, rat and other mammals. Since the SF3B4 protein sequence is highly conserved across species barriers, it is likely that the antibody is effective on many other species also.
Purity/Purification
Affinity Purified
Form/Format
PBS, 10 mM sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-SF3B4 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Full length recombinant human SF3B4 expressed in and purified from E Coli.
Immunogen Type
Recombinant protein
Immunogen
SF3B4 antibody was raised against full length recombinant human SF3B4 expressed in and purified from E Coli.
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
WB (Western Blot)
(Blots of HeLa cell crude extract stained with SF3B4 antibody. SF3B4 runs with an apparent SDS-PAGE molecular weight 49kDa.)
WB (Western Blot)
(Blots of HeLa cell crude extract stained with SF3B4 antibody. SF3B4 runs with an apparent SDS-PAGE molecular weight 49kDa.)
ICC (Immunocytochemistry)
(Human HeLa cells stained with SF3B4 antibody (red), chicken antibody to vimentin (green) and DNA (blue, stained with DAPI). The SF3B4 antibody antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.)
ICC (Immunocytochemistry)
(Human HeLa cells stained with SF3B4 antibody (red), chicken antibody to vimentin (green) and DNA (blue, stained with DAPI). The SF3B4 antibody antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.)
IHC (Immunohistochemistry)
(Anti-SF3B4 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-SF3B4 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,386 Da
NCBI Official Full Name
splicing factor 3B subunit 4
NCBI Official Synonym Full Names
splicing factor 3b, subunit 4, 49kDa
NCBI Official Symbol
SF3B4
NCBI Official Synonym Symbols
AFD1; Hsh49; SAP49; SF3b49
NCBI Protein Information
splicing factor 3B subunit 4; SAP 49; SF3b50; spliceosomal protein; spliceosome-associated protein 49; splicing factor 3b, subunit 4, 49kD; spliceosome-associated protein (U2 snRNP); pre-mRNA-splicing factor SF3b 49 kDa subunit
UniProt Protein Name
Splicing factor 3B subunit 4
UniProt Gene Name
SF3B4
UniProt Synonym Gene Names
SAP49; SAP 49
UniProt Entry Name
SF3B4_HUMAN
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Product Notes
The SF3B4 sf3b4 (Catalog #AAA52531) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 3A1] (IgG2b,k) to Human SF3B4 reacts with Bovine, Human, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SF3B4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SF3B4 sf3b4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF3B4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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