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WB (Western Blot)
(Detection of human and mouse SF3B4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), Jurkat (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-SF3B4 antibody AAA212400 (lot AAA212400-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse SF3B4 Polyclonal Antibody | anti-SF3B4 antibody
Rabbit anti-SF3B4 Antibody, Affinity Purified
Gene Names
SF3B4; AFD1; Hsh49; SAP49; SF3b49
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SF3B4, Antibody; Rabbit anti-SF3B4 Antibody, Affinity Purified; SF3b50; spliceosome-associated protein (U2 snRNP); splicing factor 3b, subunit 4, 49kDa; splicing factor 3b, subunit 4, 49kD; spliceosome-associated protein 49; spliceosomal protein; SAP49; AFD1; SAP 49; splicing factor 3B subunit 4; pre-mRNA-splicing factor SF3b 49 kDa subunit; Hsh49; SF3b49; Splicing factor 3B subunit 4; splicing factor 3b subunit 4; anti-SF3B4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SF3B4 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 374 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse SF3B4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), Jurkat (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-SF3B4 antibody AAA212400 (lot AAA212400-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse SF3B4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), Jurkat (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-SF3B4 antibody AAA212400 (lot AAA212400-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human SF3B4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-SF3B4 antibody AAA212400 (lot AAA212400-1) used for IP at 6 ug/mg lysate. SF3B4 was also immunoprecipitated by rabbit anti-SF3B4 antibody BL14223. For blotting immunoprecipitated SF3B4, AAA212400 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human SF3B4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-SF3B4 antibody AAA212400 (lot AAA212400-1) used for IP at 6 ug/mg lysate. SF3B4 was also immunoprecipitated by rabbit anti-SF3B4 antibody BL14223. For blotting immunoprecipitated SF3B4, AAA212400 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohiostchemistry)
(Detection of human SF3B4 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SF3B4 (Cat. No. AAA212400 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human SF3B4 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SF3B4 (Cat. No. AAA212400 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse SF3B4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SF3B4 (Cat. No. AAA212400 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse SF3B4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SF3B4 (Cat. No. AAA212400 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-SF3B4 antibody
Splicing factor 3b, subunit 4, 49kDa (SF3B4) is one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B [taken from NCBI Entrez Gene (Gene ID: 10262)].
Product Categories/Family for anti-SF3B4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,386 Da
NCBI Official Full Name
splicing factor 3B subunit 4
NCBI Official Synonym Full Names
splicing factor 3b, subunit 4, 49kDa
NCBI Official Symbol
SF3B4
NCBI Official Synonym Symbols
AFD1; Hsh49; SAP49; SF3b49
NCBI Protein Information
splicing factor 3B subunit 4; SAP 49; SF3b50; spliceosomal protein; spliceosome-associated protein 49; spliceosome-associated protein (U2 snRNP); pre-mRNA-splicing factor SF3b 49 kDa subunit
UniProt Protein Name
Splicing factor 3B subunit 4
UniProt Gene Name
SF3B4
UniProt Synonym Gene Names
SAP49; SAP 49
UniProt Entry Name
SF3B4_HUMAN
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Product Notes
The SF3B4 sf3b4 (Catalog #AAA212400) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SF3B4 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SF3B4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SF3B4 sf3b4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF3B4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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