IP (Immunoprecipitation)
(SF3B4 was immunoprecipitated using:; Lane A:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-SF3B4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-SF3B4 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 49 kDa; Observed band size :51 kDa)
Rabbit anti-Human SF3B4 Polyclonal Antibody | anti-SF3B4 antibody
SF3B4 Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
SF3B4, Antibody; SF3B4 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-AFD1 Antibody; Anti-Hsh49 Antibody; Anti-SAP49 Antibody; Anti-SF3b49 Antibody; anti-SF3B4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human SF3B4
Applicable Applications for anti-SF3B4 antibody
IP (Immunoprecipitation), WB (Western Blot)
Preparation
Produced in rabbits immunized with E Coli-derived Human SF3B4 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SF3B4 was immunoprecipitated using:; Lane A:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-SF3B4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-SF3B4 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 49 kDa; Observed band size :51 kDa)
IP (Immunoprecipitation)
(SF3B4 was immunoprecipitated using:; Lane A:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-SF3B4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-SF3B4 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 49 kDa; Observed band size :51 kDa)
WB (Western Blot)
(Anti-SF3B4 rabbit polyclonal antibody at 1:500 dilution; Lane A: Hela Whole Cell Lysate; Lane B: 293T Whole Cell Lysate; Lane C: Jurkat Whole Cell Lysate; Lane D: A431 Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:44 kDa; Observed band size:51 kDa)
WB (Western Blot)
(Anti-SF3B4 rabbit polyclonal antibody at 1:500 dilution; Lane A: Hela Whole Cell Lysate; Lane B: 293T Whole Cell Lysate; Lane C: Jurkat Whole Cell Lysate; Lane D: A431 Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:44 kDa; Observed band size:51 kDa)
Related Product Information for anti-SF3B4 antibody
Splicing factor 3b subunit 4 (SF3B4) is a splicing factor and potential oncogene in hepatocellular carcinoma (HCC). SF3B4 plays a crucial role in hepatocellular carcinoma (HCC) and is negatively regulated by miRNA-133b. The miRNA-133b/ SF3B4 axis may serve as a new therapeutic target for HCC treatment. SF3b4 encodes a core subunit of the U2-type spliceosome, loss- or gain-of-function of which often associates with abnormal cell growth, leading to tumorigenesis. Homologs of SF3b4 in other phyla are also essential. Haploinsufficiency of the SF3B4 gene has been identified as a significant reason for Nager syndrome. Mutations in the SF3B4 gene, which encodes a component of the pre-mRNA spliceosomal complex, were identified as a cause of Nager syndrome, accounting for 60% of affected individuals.
Product Categories/Family for anti-SF3B4 antibody
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Product Notes
The SF3B4 (Catalog #AAA258698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SF3B4 Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SF3B4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SF3B4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF3B4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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