IP (Immunoprecipitation)
(SFPQ was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with AAA62322 of 10ug. Western blot was performed from the immunoprecipitate using AAA62322 at 1/2000 dilution. Anti-Mouse-IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.Lane 1: Jurkat cells membrane fraction.Lane 2: IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration: 5% milk/TBST.)
Mouse anti-Homo sapiens SFPQ Monoclonal Antibody | anti-SFPQ antibody
SFPQ (578) Mouse mAb
Gene Names
SFPQ; PSF; POMP100; PPP1R140
Reactivity
Homo sapiens
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
SFPQ, Antibody; SFPQ (578) Mouse mAb; PPP1R140, PSF; anti-SFPQ antibody
Host
Mouse
Reactivity
Homo sapiens
Clonality
Monoclonal
Isotype
IgG
Applicable Applications for anti-SFPQ antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Host Note
This monoclonal antibody is produced by immunizing mice with a polypeptide corresponding to SFPQ protein.
Product Advantage
Mass spectrum approved
Preparation and Storage
Store at -20°C, stable for one year from date of shipment.
IP (Immunoprecipitation)
(SFPQ was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with AAA62322 of 10ug. Western blot was performed from the immunoprecipitate using AAA62322 at 1/2000 dilution. Anti-Mouse-IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.Lane 1: Jurkat cells membrane fraction.Lane 2: IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration: 5% milk/TBST.)
IP (Immunoprecipitation)
(SFPQ was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with AAA62322 of 10ug. Western blot was performed from the immunoprecipitate using AAA62322 at 1/2000 dilution. Anti-Mouse-IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.Lane 1: Jurkat cells membrane fraction.Lane 2: IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration: 5% milk/TBST.)
WB (Western Blot)
(All lanes: SFPQ Mouse mAb at 1/2000 dilutionLane 1: Jurkat cells membrane fractionLane 2: THP1 cells membrane fractionLysates/proteins at 20ug per laneSecondary: Goat anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size: 100 KDaObserved band size: 100 KDaBlocking/Dilution buffer: 1X TBST)
WB (Western Blot)
(All lanes: SFPQ Mouse mAb at 1/2000 dilutionLane 1: Jurkat cells membrane fractionLane 2: THP1 cells membrane fractionLysates/proteins at 20ug per laneSecondary: Goat anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size: 100 KDaObserved band size: 100 KDaBlocking/Dilution buffer: 1X TBST)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling SFPQ with AAA62322 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, red). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as a secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling SFPQ with AAA62322 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, red). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as a secondary antibody.)
Related Product Information for anti-SFPQ antibody
DNA and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in a spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatory elements which represses exon inclusion.
Product Categories/Family for anti-SFPQ antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 100 KDa
NCBI Official Full Name
splicing factor, proline- and glutamine-rich
NCBI Official Synonym Full Names
splicing factor proline and glutamine rich
NCBI Official Symbol
SFPQ
NCBI Official Synonym Symbols
PSF; POMP100; PPP1R140
NCBI Protein Information
splicing factor, proline- and glutamine-rich
UniProt Protein Name
Splicing factor, proline- and glutamine-rich
UniProt Gene Name
SFPQ
UniProt Synonym Gene Names
PSF; PSF1 PublicationManual assertion based on opinion iniRef.1; PTB-associated-splicing factor
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Product Notes
The SFPQ sfpq (Catalog #AAA62322) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SFPQ (578) Mouse mAb reacts with Homo sapiens and may cross-react with other species as described in the data sheet. AAA Biotech's SFPQ can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SFPQ sfpq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFPQ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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