Rabbit anti-Human, Mouse PSF/SFPQ Polyclonal Antibody | anti-PSF/SFPQ antibody

Rabbit anti-PSF/SFPQ Antibody, Affinity Purified

Cat. Num. AAA211418

• Gene Names: SFPQ; PSF; POMP100; PPP1R140
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: PSF/SFPQ, Antibody; Rabbit anti-PSF/SFPQ Antibody, Affinity Purified; protein phosphatase 1, regulatory subunit 140; DNA-binding p52/p100 complex, 100 kDa subunit; splicing factor, proline- and glutamine-rich; splicing factor proline/glutamine-rich; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); PTB-associated-splicing factor; PTB-associated splicing factor; PSF; PPP1R140; POMP100; Polypyrimidine tract-binding protein-associated-splicing factor; polypyrimidine tract-binding protein-associated splicing factor; polypyrimidine tract binding protein associated; epididymis secretory sperm binding protein; 100 kDa DNA-pairing protein; hPOMp100; Splicing factor, proline- and glutamine-rich; splicing factor proline and glutamine rich; anti-PSF/SFPQ antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-PSF/SFPQ antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 225 and 275
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse PSF/SFPQ by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSF/SFPQ antibody AAA211418 (lot AAA211418-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)

IP (Immunoprecipitation)

(Detection of human PSF/SFPQ by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PSF/SFPQ antibody AAA211418 (lot AAA211418-1) used for IP at 3 ug/mg lysate. PSF/SFPQ was also immunoprecipitated by rabbit anti-PSF/SFPQ antibodies and which recognize other epitopes. For blotting immunoprecipitated PSF/SFPQ, AAA211418 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 1 second.)

IHC (Immunohistochemistry)

(Detection of human PSF/SFPQ by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PSF/SFPQ (Cat. No. AAA211418 lot 1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)

Related Product Information for anti-PSF/SFPQ antibody

PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3' processing and transcription termination.

Product Categories/Family for anti-PSF/SFPQ antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4826998
• NCBI GeneID: 6421
• NCBI Accession #: NP_005057.1
• NCBI GenBank Nucleotide #: NM_005066.2
• UniProt Accession #: P23246; P30808; Q5SZ71
• Molecular Weight: 72,263 Da
• NCBI Official Full Name: splicing factor, proline- and glutamine-rich
• NCBI Official Synonym Full Names: splicing factor proline/glutamine-rich
• NCBI Official Symbol: SFPQ
• NCBI Official Synonym Symbols: PSF; POMP100; PPP1R140
• NCBI Protein Information: splicing factor, proline- and glutamine-rich; hPOMp100; 100 kDa DNA-pairing protein; PTB-associated splicing factor; PTB-associated-splicing factor; DNA-binding p52/p100 complex, 100 kDa subunit; protein phosphatase 1, regulatory subunit 140; polypyrimidi
• UniProt Protein Name: Splicing factor, proline- and glutamine-rich
• UniProt Gene Name: SFPQ
• UniProt Synonym Gene Names: PSF; hPOMp100; PSF; PTB-associated-splicing factor
• UniProt Entry Name: SFPQ_HUMAN