SOX10 recombinant antibody

Rabbit anti-SOX10 Recombinant Monoclonal Antibody [BLR080G]

Cat. Num. AAA23824

• Gene Names: SOX10; DOM; WS4; PCWH; WS2E; WS4C
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Purified
• Synonyms: SOX10, Recombinant Antibody; Rabbit anti-SOX10 Recombinant Monoclonal Antibody [BLR080G]; transcription factor SOX-10; SRY-related HMG-box gene 10; WS4C; WS4; SRY (sex determining region Y)-box 10; PCWH; dominant megacolon, mouse, human homolog of; DOM; WS2E; Transcription factor SOX-10; SRY-box 10; SOX10 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR080G
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 50ug/ml (varies by lot)

• Applicable Applications for SOX10 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Multiplex immunofluorescence (mIF)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; mIF: 1:250
• Immunogen: Between 416 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse SOX10 by western blot. Samples: Whole cell lysate from TCMK-1 (50 ug), B16-F10 (15 ug), NIH 3T3 (50 ug), and Malme-3M (15 ug) cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

WB (Western Blot)

(Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 ug) from HEK293T, Malme-3M, MCF-7, SK-MEL-28, Jurkat, MDA-MB-435, HeLa, and UACC-62 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation)

(Detection of human SOX10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Malme-3M cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1) used for IP at 20 ul/mg lysate. SOX10 was also immunoprecipitated by a rabbit anti-SOX10 antibodies . For blotting immunoprecipitated SOX10, AAA23824 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry)

(Detection of human SOX10 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human SOX10 in FFPE melanoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human SOX10 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

Related Product Information for SOX10 recombinant antibody

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. SOX10 may act as a transcriptional activator after forming a protein complex with other proteins. SOX10 acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development [taken from NCBI Entrez Gene (Gene ID: 6663)].

Product Categories/Family for SOX10 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5902104
• NCBI GeneID: 6663
• NCBI Accession #: NP_008872.1
• NCBI GenBank Nucleotide #: NM_006941.3
• UniProt Accession #: P56693; Q6FHW7; B4DV62
• Molecular Weight: 49911 MW
• NCBI Official Full Name: transcription factor SOX-10
• NCBI Official Synonym Full Names: SRY-box 10
• NCBI Official Symbol: SOX10
• NCBI Official Synonym Symbols: DOM; WS4; PCWH; WS2E; WS4C
• NCBI Protein Information: transcription factor SOX-10
• UniProt Protein Name: Transcription factor SOX-10
• UniProt Gene Name: SOX10