WB (Western Blot)
(SPARC Antibody (C-term)(Ascites) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPARC antibody detected the SPARC protein (arrow).)
Mouse SPARC Monoclonal Antibody | anti-SPARC antibody
SPARC Antibody (C-term) Antibody (Ascites)
Gene Names
SPARC; ON; BM-40
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus)
Applications
ELISA, Western Blot
Synonyms
SPARC, Antibody; SPARC Antibody (C-term) Antibody (Ascites); SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine; anti-SPARC antibody
Host
Mouse
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus)
Clonality
Monoclonal
Isotype
IgG1
Specificity
This SPARC antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.
Form/Format
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Sequence Positions
224-251
Sequence Length
303
Applicable Applications for anti-SPARC antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Cellular Location
Secreted, extracellular space, extracellular matrix, basement membrane.
Note: In or around the basement membrane
Note: In or around the basement membrane
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(SPARC Antibody (C-term)(Ascites) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPARC antibody detected the SPARC protein (arrow).)
WB (Western Blot)
(SPARC Antibody (C-term)(Ascites) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPARC antibody detected the SPARC protein (arrow).)
Related Product Information for anti-SPARC antibody
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Product Categories/Family for anti-SPARC antibody
References
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Howard, C., et al. Histol. Histopathol. 25(9):1163-1169(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Liang, J.F., et al. J. Exp. Clin. Cancer Res. 29, 71 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Howard, C., et al. Histol. Histopathol. 25(9):1163-1169(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Liang, J.F., et al. J. Exp. Clin. Cancer Res. 29, 71 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34632
NCBI Official Full Name
SPARC isoform 1
NCBI Official Synonym Full Names
secreted protein, acidic, cysteine-rich (osteonectin)
NCBI Official Symbol
SPARC
NCBI Official Synonym Symbols
ON; BM-40
NCBI Protein Information
SPARC
UniProt Protein Name
SPARC
UniProt Gene Name
SPARC
UniProt Synonym Gene Names
ON; BM-40; ON
UniProt Entry Name
SPRC_HUMAN
Similar Products
Product Notes
The SPARC sparc (Catalog #AAA283525) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 224-251. The SPARC Antibody (C-term) Antibody (Ascites) reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's SPARC can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SPARC sparc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPARC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.