Mouse secreted protein, acidic, cysteine-rich (osteonectin) ELISA Kit | SPARC elisa kit

Mouse osteonectin, ON ELISA Kit

Cat. Num. AAA80412

• Gene Names: Sparc; ON; BM-40
• Reactivity: Mouse
• Synonyms: secreted protein, acidic, cysteine-rich (osteonectin); N/A; Mouse osteonectin, ON ELISA Kit; Mouse osteonectin; ON ELISA Kit; ON; cysteine-rich protein; osteonectin; secreted protein; acidic; cysteine-rich; cysteine-rich (osteonectin); SPARC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse SPARC. No significant cross-reactivity or interference between Mouse SPARC and analogues was observed.
• Sequence Length: 302
• Detection Range: 31.2 pg/ml-2000 pg/ml
• Sensitivity: 8 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for SPARC elisa kit

Introduction: Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. Some current research shows a correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis. Osteonectin is an acidic, secreted extracellular matrix glycoprotein that plays a vital role in bone mineralization, cell-matrix interactions, and collagen binding. Osteonectin also increases the production and activity of matrix metalloproteinases, a function important to invading cancer cells within bone. Additional functions of osteonectin beneficial to tumor cells include angiogenesis, proliferation and migration. Overexpression of osteonectin is reported in many Mouse cancers such as breast, prostate and colon.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ON. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ON and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ON, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ON in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6678077
• NCBI GeneID: 20692
• NCBI Accession #: NP_033268.1
• NCBI GenBank Nucleotide #: NM_009242.5
• UniProt Accession #: P07214
• Molecular Weight: 34,450 Da
• NCBI Official Full Name: SPARC
• NCBI Official Synonym Full Names: secreted acidic cysteine rich glycoprotein
• NCBI Official Symbol: Sparc
• NCBI Official Synonym Symbols: ON; BM-40
• NCBI Protein Information: SPARC; osteonectin; basement-membrane protein 40; secreted protein acidic and rich in cysteine
• UniProt Protein Name: SPARC
• UniProt Gene Name: Sparc
• UniProt Synonym Gene Names: BM-40; ON
• UniProt Entry Name: SPRC_MOUSE