Mouse Spastin Monoclonal Antibody | anti-SPAST antibody

Spastin

Cat. Num. AAA215205

• Gene Names: SPAST; FSP2; SPG4; ADPSP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Spastin, Antibody; Spastin; ADPSP; FSP2; Spast; Spatin; SPG4; anti-SPAST antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: Sp 6C6
• Specificity: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40% of all autosomal dominant HSPs.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 551

• Applicable Applications for anti-SPAST antibody: WB (Western Blot), IF (Immunofluorescence)
• Positive Control: HeLa cells. K-562 or Raji cell lysates.
• Immunogen: Recombinant full-length human Spastin protein.
• Cellular Localization: Cell Membrane and Secreted.
• Hu-chromosome Location: 2p22.3
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Spastin Mouse Monoclonal Antibody (Sp 6C6). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot)

(Western Blot Analysis of human Raji cell lysates using Spastin Mouse Monoclonal Antibody (Sp 6C6).)

WB (Western Blot)

(Western Blot Analysis of human K-562 cell lysates using Spastin Mouse Monoclonal Antibody (Sp 6C6).)

References

Daftary et al. 2011. Mol Endocrinol. 25(9):1539-49. PMID: 21757506.

NCBI and Uniprot Product Information

• NCBI GI #: 939620306
• NCBI GeneID: 6683
• NCBI GenBank Nucleotide #: NP_001303438.1
• UniProt Accession #: Q8I0P1; Q9UBP0
• Molecular Weight: 52kDa
• NCBI Official Full Name: spastin, isoform E
• NCBI Official Synonym Full Names: spastin
• NCBI Official Symbol: SPAST
• NCBI Official Synonym Symbols: FSP2; SPG4; ADPSP
• NCBI Protein Information: spastin
• UniProt Protein Name: Spastin
• UniProt Gene Name: spas