Mouse Spectrin beta III (SPTBN2) Monoclonal Antibody | anti-SPTBN2 antibody

Spectrin beta III (SPTBN2)

Cat. Num. AAA214524

• Gene Names: SPTBN2; SCA5; SCAR14; GTRAP41
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Synonyms: Spectrin beta III (SPTBN2), Antibody; Spectrin beta III (SPTBN2); Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2; anti-SPTBN2 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: SPTBN2/1582
• Specificity: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta I (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. Spectrin beta III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 2390

• Applicable Applications for anti-SPTBN2 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Recombinant human SPTBN2 fragment (aa356-475)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 11q13.2
• Positive Control: HeLa cells. Pancreas or Liver.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded Human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1582).)

WB (Western Blot)

(Western Blot Analysis of HeLa and 293 Cell Lysates using Spectrin beta III Monoclonal Antibody (SPTBN2/1582).)

References

s Speicher, D.W. 1986. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins. J. Cell. Biochem. 30: 245-258. Coelman, T.R., et al. J.S. 1989. Functional diversity among spectrin isoforms. Cell Motil. Cytoskeleton 12: 225-247.

NCBI and Uniprot Product Information

• NCBI GI #: 5902122
• NCBI GeneID: 6712
• NCBI Accession #: NP_008877.1
• NCBI GenBank Nucleotide #: NM_006946.3
• UniProt Accession #: O15020; O14872; O14873
• Molecular Weight: 246kDa
• NCBI Official Full Name: spectrin beta chain, non-erythrocytic 2
• NCBI Official Synonym Full Names: spectrin beta, non-erythrocytic 2
• NCBI Official Symbol: SPTBN2
• NCBI Official Synonym Symbols: SCA5; SCAR14; GTRAP41
• NCBI Protein Information: spectrin beta chain, non-erythrocytic 2
• UniProt Protein Name: Spectrin beta chain, non-erythrocytic 2
• UniProt Gene Name: SPTBN2
• UniProt Synonym Gene Names: KIAA0302; SCA5