WB (Western Blot)
(Detection of human SPTBN2/SCA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN2/SCA5 antibody AAA211323 (lot AAA211323-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human SPTBN2/SCA5 Polyclonal Antibody | anti-SPTBN2/SCA5 antibody
Rabbit anti-SPTBN2/SCA5 Antibody, Affinity Purified
Gene Names
SPTBN2; SCA5; SCAR14; GTRAP41
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SPTBN2/SCA5, Antibody; Rabbit anti-SPTBN2/SCA5 Antibody, Affinity Purified; glutamate transporter EAAT4-associated protein 41; spectrin, non-erythroid beta chain 2; spectrin beta chain, non-erythrocytic 2; spectrin beta III sigma 2; spinocerebellar ataxia 5 protein; SCAR14; GTRAP41; beta-III spectrin; spectrin beta chain, brain 2; SCA5; Spectrin beta chain, non-erythrocytic 2; spectrin beta, non-erythrocytic 2; anti-SPTBN2/SCA5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SPTBN2/SCA5 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 2340 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SPTBN2/SCA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN2/SCA5 antibody AAA211323 (lot AAA211323-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human SPTBN2/SCA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN2/SCA5 antibody AAA211323 (lot AAA211323-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SPTBN2/SCA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPTBN2/SCA5 antibody AAA211323 (lot AAA211323-3) used for IP at 6 ug per reaction. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody For blotting immunoprecipitated SPTBN2/SCA5, AAA211323 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SPTBN2/SCA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPTBN2/SCA5 antibody AAA211323 (lot AAA211323-3) used for IP at 6 ug per reaction. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody For blotting immunoprecipitated SPTBN2/SCA5, AAA211323 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-SPTBN2/SCA5 antibody
SPTBN2/SCA5 is a member of the beta spectrin gene family and a subunit of the spectrin cytoskeletal scaffolding protein that links the plasma membrane to cytoskeletal actin and functions in cellular shape and organelle organization. SPTBN2/SCA5 has been identified as a Golgi- and vesicle-associated spectrin. Mutations in SPTBN2/SCA5 cause spinocerebellar ataxia type 5 (SCA5), an autosomal dominant disease characterized by progressive incoordination of gait, hand, speech, and eye movements.
Product Categories/Family for anti-SPTBN2/SCA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
268,309 Da
NCBI Official Full Name
spectrin beta chain, non-erythrocytic 2
NCBI Official Synonym Full Names
spectrin beta, non-erythrocytic 2
NCBI Official Symbol
SPTBN2
NCBI Official Synonym Symbols
SCA5; SCAR14; GTRAP41
NCBI Protein Information
spectrin beta chain, non-erythrocytic 2
UniProt Protein Name
Spectrin beta chain, non-erythrocytic 2
UniProt Gene Name
SPTBN2
UniProt Synonym Gene Names
KIAA0302; SCA5
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Product Notes
The SPTBN2/SCA5 sptbn2 (Catalog #AAA211323) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SPTBN2/SCA5 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPTBN2/SCA5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SPTBN2/SCA5 sptbn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPTBN2/SCA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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