Mouse SULFATASE 2 Monoclonal Antibody | anti-Sulf2 antibody

MOUSE ANTI HUMAN SULFATASE 2 (C-TERMINAL)

Cat. Num. AAA49794

• Gene Names: Sulf2; MSulf-2; AU020235; mKIAA1247; 2010004N24Rik
• Reactivity: Mouse; N.B Antibody reactivity and working conditions may vary between species.
• Applications: Western Blot, Immunohistochemistry, ELISA
• Synonyms: SULFATASE 2, Antibody; MOUSE ANTI HUMAN SULFATASE 2 (C-TERMINAL); SULF-2, KIAA1247; anti-Sulf2 antibody

• Host: Mouse
• Reactivity: Mouse; N.B Antibody reactivity and working conditions may vary between species.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2B4
• Specificity: SULFATASE 2(C-Terminal)
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 875

• Applicable Applications for anti-Sulf2 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Preservative Stabilisers: 0.09% Sodium Azide.
• Preparation: Purified IgG prepared by affinity chromatography on Protein G.
• Immunogen: Recombinant human Sulf-2.<
• Histology Positive Control Tissue: Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
• Target Species: Human
• Buffer Solution: Phosphate buffered saline.
• Preparation and Storage: Store at 4°C or at -20°C if preferred. ; Storage in frost-free freezers is not recommended.; This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.; ; Should this product contain a precipitate we recommend microcentrifugation before use.; ; Shelf Life: 18 months from date of despatch.

Application Data

(Paraffin embedded HEK293 cells transfected with SULF2 stained with Mouse anti Human Sulfatase 2 C-terminal)

Related Product Information for anti-Sulf2 antibody

Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Studies have linked Sulf-2 with several cancers, including breast, hepatocellular, and pancreatic cancer, and recently with non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010). Mature human Sulf-2 is a heterodimer consisting of a 50kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75kDa N-terminal subunit which contains the catalytic centre. Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.

NCBI and Uniprot Product Information

• NCBI GI #: 357588451
• NCBI GeneID: 72043
• NCBI Accession #: NP_001239507.1
• NCBI GenBank Nucleotide #: NM_001252578.1
• NCBI Official Full Name: extracellular sulfatase Sulf-2 isoform 1
• NCBI Official Synonym Full Names: sulfatase 2
• NCBI Official Symbol: Sulf2
• NCBI Official Synonym Symbols: MSulf-2; AU020235; mKIAA1247; 2010004N24Rik
• NCBI Protein Information: extracellular sulfatase Sulf-2
• UniProt Protein Name: Extracellular sulfatase Sulf-2
• UniProt Gene Name: Sulf2
• UniProt Synonym Gene Names: mSulf-2
• UniProt Entry Name: SULF2_MOUSE