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Mouse TCR alpha/beta Monoclonal Antibody | anti-TCRalpha/beta antibody

Anti-Rt TCR alpha/beta Purified Low Endotoxin

Reactivity
Non-human primates, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay, Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified by protein-A affinity chromatography
Synonyms
TCR alpha/beta, Antibody; Anti-Rt TCR alpha/beta Purified Low Endotoxin; TCRA/B; anti-TCRalpha/beta antibody
Ordering
Host
Mouse
Reactivity
Non-human primates, Rat
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
R73
Specificity
The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
Purity/Purification
Purified by protein-A affinity chromatography
Form/Format
Purified Low Endotoxin. Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TCRalpha/beta antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), FA (Functional Assay), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Rat T blasts and erythrocytes
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.
Related Product Information for anti-TCRalpha/beta antibody
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form Functional Application (FUNC)tional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
References
*Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8921942,*Kerstan A, Hünig T: Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells. J Immunol. 2004 Feb 1;172(3):1341-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/14734708,*Carlson BC, Jansson AM, Larsson A, Bucht A, Lorentzen JC: The endogenous adjuvant squalene can induce a chronic T-cell-mediated arthritis in rats. Am J Pathol. 2000 Jun;156(6):2057-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/10854227,*Shao H, Sun SL, Kaplan HJ, Sun D: Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal-binding protein 1177-1191: J Immunol. 2004 Aug 15;173(4):2849-54., URL: https://www.ncbi.nlm.nih.gov/pubmed/15295004,*Li S, Thanikachalam M, Pang M, Kawaharada N, Aitouche A, Pham SM: A clinically relevant CTLA4-Ig-based regimen induces chimerism and tolerance to heart grafts. Ann Thorac Surg. 2001 Oct;72(4):1306-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/11603451,*Müller N, van den Brandt J, Odoardi F, Tischner D, Herath J, Flügel A, Reichardt HM: A CD28 superagonistic antibody elicits 2 Functional Application (FUNC)tionally distinct waves of T cell activation in rats. J Clin Invest. 2008 Apr;118(4):1405-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/18357346,*Hansson AS, Heinegård D, Holmdahl R: A new animal model for relapsing polychondritis, induced by cartilage matrix protein (matrilin-1). J Clin Invest. 1999 Sep;104(5):589-98., URL: https://www.ncbi.nlm.nih.gov/pubmed/10487773,*Wilhelm MJ, Pratschke J, Beato F, Taal M, Kusaka M, Hancock WW, Tilney NL: Activation of the heart by donor brain death accelerates acute rejection after transplantation. Circulation. 2000 Nov 7;102(19):2426-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/11067799,*Kreiss M, Asmuss A, Krejci K, Lindemann D, Miyoshi-Akiyama T, Uchiyama T, Rink L, Broeren CP, Herrmann T: Contrasting contributions of complementarity-determining region 2 and hypervariable region 4 of rat BV8S2+ (Vbeta8.2) TCR to the recognition of myelin basic protein and different types of bacterial superantigens. Int Immunol. 2004 May;16(5):655-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/15096488,*Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G: A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989 Jan 1;169(1):73-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/2783336,*Fuller JM, Bogdani M, Tupling TD, Jensen RA, Pefley R, Manavi S, Cort L, Blankenhorn EP, Mordes JP, Lernmark A, Kwitek AE: Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene. Physiol Genomics. 2009 Jun 10;38(1):89-97., URL: https://www.physiology.org/doi/full/10.1152/physio,*Ritter T, Schröder G, Risch K, Vergopoulos A, Shean MK, Kolls J, Brock J, Lehmann M, Volk HD: Ischemia/reperfusion injury-mediated down-regulation of adenovirus-mediated gene expression in a rat heart transplantation model is inhibited by co-application of a TNFRp55-Ig chimeric construct. Gene Ther. 2000 Jul;7(14):1238-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/10918493,*Radojevic K, Arsenovic-Ranin N, Kosec D, Pesic V, Pilipovic I, Perisic M, Plecas-Solarovic B, Leposavic G: Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level. J Endocrinol. 2007 Mar;192(3):669-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/17332534

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Product Notes

The TCRalpha/beta (Catalog #AAA129124) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rt TCR alpha/beta Purified Low Endotoxin reacts with Non-human primates, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TCR alpha/beta can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), FA (Functional Assay), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TCRalpha/beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR alpha/beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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