Mouse Thrombomodulin/CD141 Monoclonal Antibody | anti-THBD antibody

Thrombomodulin/CD141 (Endothelial Cell Marker)

Cat. Num. AAA214532

• Gene Names: THBD; TM; THRM; AHUS6; BDCA3; CD141; THPH12
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Synonyms: Thrombomodulin/CD141, Antibody; Thrombomodulin/CD141 (Endothelial Cell Marker); AHUS6; BDCA3; CD141; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin (TM); anti-THBD antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: THBD/1591
• Specificity: It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposis sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 575

• Applicable Applications for anti-THBD antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Recombinant human Thrombomodulin protein fragment (aa69-194)
• Cellular Localization: Cell Surface & Cytoplasmic
• Hu-chromosome Location: 20p11.2
• Positive Control: THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Thrombomodulin Monoclonal/CD141 Antibody (THBD/1591).)

WB (Western Blot)

(Western Blot Analysis (A) Recombinant Protein (B) THP1 Cell lysate Using Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).)

References

Appleton MA, Attanoos RL, Jasani B. Thrombomodulin as a marker of vascular and lymphatic tumours. Histopathology. 1996 Aug; 29(2):153-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4507483
• NCBI GeneID: 7056
• NCBI Accession #: NP_000352.1
• NCBI GenBank Nucleotide #: NM_000361.2
• UniProt Accession #: P07204; Q8IV29; Q9UC32
• Molecular Weight: ~100kDa
• NCBI Official Full Name: thrombomodulin
• NCBI Official Synonym Full Names: thrombomodulin
• NCBI Official Symbol: THBD
• NCBI Official Synonym Symbols: TM; THRM; AHUS6; BDCA3; CD141; THPH12
• NCBI Protein Information: thrombomodulin
• UniProt Protein Name: Thrombomodulin
• UniProt Gene Name: THBD
• UniProt Synonym Gene Names: THRM; TM