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WB (Western Blot)
(Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human Myc/DDK- tagged TIM-3 (hTIM-3-Myc/DDK; +), using TIM-3 Rabbit mAb.)
Rabbit anti-Human TIM-3 (T Cell Immunoglobulin and Mucin Domain-containing 3, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) Monoclonal Antibody | anti-TIM-3 antibody
TIM-3 (T Cell Immunoglobulin and Mucin Domain-containing 3, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3)
Gene Names
HAVCR2; TIM3; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
TIM-3 (T Cell Immunoglobulin and Mucin Domain-containing 3, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3), Antibody; TIM-3 (T Cell Immunoglobulin and Mucin Domain-containing 3, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3); anti-TIM-3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
D5D5R
Specificity
Recognizes endogenous levels of total human TIM-3.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol
Applicable Applications for anti-TIM-3 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Flow Cytometry: 1:50
Western Blot: 1:1000,. Incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:400 Requires HIER using EDTA, pH 8.0 prior to staining.
Immunoprecipitation: 1:50
Western Blot: 1:1000,. Incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:400 Requires HIER using EDTA, pH 8.0 prior to staining.
Immunoprecipitation: 1:50
Immunogen
Recombinant protein specific to the extracellular domain to human TIM-3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
WB (Western Blot)
(Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human Myc/DDK- tagged TIM-3 (hTIM-3-Myc/DDK; +), using TIM-3 Rabbit mAb.)
WB (Western Blot)
(Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human Myc/DDK- tagged TIM-3 (hTIM-3-Myc/DDK; +), using TIM-3 Rabbit mAb.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TIM-3 Rabbit mAb. Note staining of alveolar macrophages.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TIM-3 Rabbit mAb. Note staining of alveolar macrophages.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using AAA26909. Controls were run on Jurkat cells (orange) and primary CD4+ T cells (red) using concentration-matched Rabbit mAb IgG Isotype Control. Anti-rabbit IgG (H+L), F(ab’)2 fragment was used as a secondary antibody. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml))
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using AAA26909. Controls were run on Jurkat cells (orange) and primary CD4+ T cells (red) using concentration-matched Rabbit mAb IgG Isotype Control. Anti-rabbit IgG (H+L), F(ab’)2 fragment was used as a secondary antibody. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml))
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil using AAA26909.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil using AAA26909.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded cell pellets, primary CD4+ T cells (left) and HT-29 cells (right), using AAA26909 T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded cell pellets, primary CD4+ T cells (left) and HT-29 cells (right), using AAA26909 T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma using AAA26909.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma using AAA26909.)
WB (Western Blot)
(Western Blot analysis of extracts from primary human CD4+ T cells and various cell lines using AAA26909 (upper) or B-Actin Rabbit mAb (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml).)
WB (Western Blot)
(Western Blot analysis of extracts from primary human CD4+ T cells and various cell lines using AAA26909 (upper) or B-Actin Rabbit mAb (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml).)
Related Product Information for anti-TIM-3 antibody
T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer. In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease. Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death.
Product Categories/Family for anti-TIM-3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
301
NCBI Official Full Name
Hepatitis A virus cellular receptor 2
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 2
NCBI Official Symbol
HAVCR2
NCBI Official Synonym Symbols
TIM3; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
NCBI Protein Information
hepatitis A virus cellular receptor 2; kidney injury molecule-3; T-cell membrane protein 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin mucin receptor 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglo
UniProt Protein Name
Hepatitis A virus cellular receptor 2
UniProt Gene Name
HAVCR2
UniProt Synonym Gene Names
TIM3; TIMD3; HAVcr-2; TIMD-3; TIM-3
UniProt Entry Name
HAVR2_HUMAN
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Product Notes
The TIM-3 havcr2 (Catalog #AAA26909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIM-3 (T Cell Immunoglobulin and Mucin Domain-containing 3, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIM-3 (T Cell Immunoglobulin and Mucin Domain-containing 3, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Flow Cytometry: 1:50 Western Blot: 1:1000,. Incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:400 Requires HIER using EDTA, pH 8.0 prior to staining. Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the TIM-3 havcr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIM-3 (T Cell Immunoglobulin and Mucin Domain-containing 3, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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