Mouse anti-Human Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Monoclonal Antibody | anti-TOP1MT antibody

Topoisomerase (DNA) I, Mitochondrial (TOP1MT)

Cat. Num. AAA214895

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Topoisomerase (DNA) I, Mitochondrial (TOP1MT), Antibody; Topoisomerase (DNA) I, Mitochondrial (TOP1MT); DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase; anti-TOP1MT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: TOP1MT/568
• Specificity: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 503

• Applicable Applications for anti-TOP1MT antibody: IHC (Immunohistochemistry)
• Positive Control: A431 cells. Heart, Skeletal muscle, brain or fetal liver.
• Immunogen: Recombinant full-length human TOP1MT protein
• Cellular Localization: Cytoplasmic (Mitochondria)
• Hu-chromosome Location: 8q24.3
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Topo I, MT Mouse Monoclonal Antibody (TOP1MT/568). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Mouse Monoclonal Antibody (TOP1MT/568))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Mouse Monoclonal Antibody (TOP1MT/568).)

References

1. Zhang, H., et al. 2001. Proc. Natl. Acad. Sci. USA 98: 10608-10613. 2. Zhang, H., et al. 2007. Biochimie 89: 474-481

NCBI and Uniprot Product Information

• NCBI GI #: 386642871
• NCBI GeneID: 116447
• NCBI Accession #: NP_001245375.1
• NCBI GenBank Nucleotide #: NP_001245375.1
• UniProt Accession #: Q969P6
• Molecular Weight: 70kDa
• NCBI Official Full Name: DNA topoisomerase I, mitochondrial isoform 2
• NCBI Official Synonym Full Names: DNA topoisomerase I mitochondrial
• NCBI Official Symbol: TOP1MT
• NCBI Protein Information: DNA topoisomerase I, mitochondrial
• UniProt Protein Name: DNA topoisomerase I, mitochondrial
• UniProt Gene Name: TOP1MT
• UniProt Synonym Gene Names: TOP1mt
• UniProt Entry Name: TOP1M_HUMAN