IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human liver stained with Topo I, MT Recombinant Mouse Monoclonal Antibody (rTOP1MT/488).)
Mouse anti-Human Topoisomerase I, Mitochondrial (TOP1MT) Monoclonal Antibody | anti-TOP1MT antibody
Topoisomerase I, Mitochondrial (TOP1MT) Recombinant Mouse Monoclonal Antibody [Clone rTOP1MT/488]
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Topoisomerase I, Mitochondrial (TOP1MT), Antibody; Topoisomerase I, Mitochondrial (TOP1MT) Recombinant Mouse Monoclonal Antibody [Clone rTOP1MT/488]; DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase; anti-TOP1MT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse/IgG1, kappa
Clone Number
rTOP1MT/488
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-TOP1MT antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant full-length human TOP1MT protein
Cellular Localization
Cytoplasm (Mitochondria)
Positive Control
MCF-7 or A431 cells. Jurkat cell lysate. Human heart, skeletal muscle, brain or fetal liver tissue.
Hu-chromosome Location
8q24.3
Unigene
528574
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human liver stained with Topo I, MT Recombinant Mouse Monoclonal Antibody (rTOP1MT/488).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human liver stained with Topo I, MT Recombinant Mouse Monoclonal Antibody (rTOP1MT/488).)
Related Product Information for anti-TOP1MT antibody
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70kDa
NCBI Official Full Name
DNA topoisomerase I, mitochondrial isoform 2
NCBI Official Synonym Full Names
topoisomerase (DNA) I, mitochondrial
NCBI Official Symbol
TOP1MT
NCBI Protein Information
DNA topoisomerase I, mitochondrial
UniProt Protein Name
DNA topoisomerase I, mitochondrial
UniProt Gene Name
TOP1MT
UniProt Synonym Gene Names
TOP1mt
UniProt Entry Name
TOP1M_HUMAN
Similar Products
Product Notes
The TOP1MT top1mt (Catalog #AAA215548) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Topoisomerase I, Mitochondrial (TOP1MT) Recombinant Mouse Monoclonal Antibody [Clone rTOP1MT/488] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Topoisomerase I, Mitochondrial (TOP1MT) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TOP1MT top1mt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topoisomerase I, Mitochondrial (TOP1MT), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.