IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded H441 Xenograft using DNA ligase 3 antibody at a dilution of 1:100)
Rabbit DNA ligase 3 Polyclonal Antibody | anti-DNA antibody
DNA ligase 3 antibody
Gene Names
LIG3; LIG2
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
DNA ligase 3 antibody was purified by antigen-affinity chromatography
Synonyms
DNA ligase 3, Antibody; DNA ligase 3 antibody; Polyclonal DNA ligase 3; Anti-DNA ligase 3; DNA ligase 3; ligase II DNA ATP-dependent; LIG3; ligase III DNA ATP-dependent; DNA ligase -3; LIG2; polydeoxyribonucleotide synthase [ATP] 3; anti-DNA antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
DNA ligase 3 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.31 mg/ml (varies by lot)
Sequence Length
949
Applicable Applications for anti-DNA antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Biological Significance
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus.
Cross-Reactivity
Human
Immunogen
DNA ligase 3 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 765 and 1009 of DNA ligase 3
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded H441 Xenograft using DNA ligase 3 antibody at a dilution of 1:100)
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded H441 Xenograft using DNA ligase 3 antibody at a dilution of 1:100)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: Molt-4) using a 7.5 % SDS PAGE gel and DNA ligase 3 antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: Molt-4) using a 7.5 % SDS PAGE gel and DNA ligase 3 antibody at a dilution of 1:1000)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using DNA ligase 3 antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using DNA ligase 3 antibody at 1:200 dilution.)
Related Product Information for anti-DNA antibody
Affinity purified Rabbit polyclonal DNA ligase 3 antibody
Product Categories/Family for anti-DNA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113 kDa
NCBI Official Full Name
DNA ligase 3 isoform beta
NCBI Official Synonym Full Names
ligase III, DNA, ATP-dependent
NCBI Official Symbol
LIG3
NCBI Official Synonym Symbols
LIG2
NCBI Protein Information
DNA ligase 3
UniProt Protein Name
DNA ligase 3
UniProt Gene Name
LIG3
UniProt Entry Name
DNLI3_HUMAN
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Product Notes
The DNA lig3 (Catalog #AAA107888) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNA ligase 3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the DNA lig3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA ligase 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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