IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).)
Mouse Topoisomerase II alpha Monoclonal Antibody | anti-TOP2A antibody
Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human. Others not known.
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Topoisomerase II alpha, Antibody; Topoisomerase II alpha (Proliferation & Drug-Resistance Marker); ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; DNA Topoisomerase2; TOP2A; Topoisomerase DNA II alpha 170kDa; TP2A; anti-TOP2A antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
TOP2A/1362
Specificity
It recognizes a 170kDa protein, which is identified as topoisomerase II. shows no cross-reaction with Topoisomerase II or I. Topo II plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIï¡ is also implicated in drug resistance of tumor cells.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
1531
Applicable Applications for anti-TOP2A antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
Cellular Localization
Nuclear
Hu-chromosome Location
17q21.2
Positive Control
HeLa cells or Tonsil
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).)
References
Chung, T.D., et al. 1989. Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes. Proc. Natl. Acad. Sci USA 86: 9431-9435.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
170kDa
NCBI Official Full Name
DNA topoisomerase 2-alpha
NCBI Official Synonym Full Names
DNA topoisomerase II alpha
NCBI Official Symbol
TOP2A
NCBI Official Synonym Symbols
TOP2; TP2A
NCBI Protein Information
DNA topoisomerase 2-alpha
UniProt Protein Name
DNA topoisomerase 2-alpha
UniProt Gene Name
TOP2A
UniProt Synonym Gene Names
TOP2
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Product Notes
The TOP2A top2a (Catalog #AAA214541) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Topoisomerase II alpha can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topoisomerase II alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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