IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/6629).)
Mouse Topoisomerase II alpha Monoclonal Antibody | anti-TOP2A antibody
Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Recombinant Mouse Monoclonal Antibody [Clone rTOP2A/6629]
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human and Mouse.
Applications
Immunohistochemistry
Synonyms
Topoisomerase II alpha, Antibody; Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Recombinant Mouse Monoclonal Antibody [Clone rTOP2A/6629]; Topoisomerase II alpha (Proliferation & Drug-Resistance Marker); ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; DNA Topoisomerase2; TOP2A; Topoisomerase DNA II alpha 170kDa; TP2A; anti-TOP2A antibody
Host
Mouse
Reactivity
Human and Mouse.
Clonality
Monoclonal
Isotype
Mouse/IgG1, kappa
Clone Number
rTOP2A/6629
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-TOP2A antibody
IHC (Immunohistochemistry)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
Cellular Localization
Nuclear
Positive Control
Human lymph node or tonsil.
Hu-chromosome Location
17q21.2
Unigene
156346
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/6629).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/6629).)
Related Product Information for anti-TOP2A antibody
It recognizes a 170kDa protein, which is identified as topoisomerase IIalpha. It shows no cross-reaction with Topoisomerase II beta or I. Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II alpha is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
174,385 Da
NCBI Official Full Name
DNA topoisomerase 2-alpha
NCBI Official Synonym Full Names
topoisomerase (DNA) II alpha 170kDa
NCBI Official Symbol
TOP2A
NCBI Official Synonym Symbols
TOP2; TP2A
NCBI Protein Information
DNA topoisomerase 2-alpha; DNA gyrase; DNA topoisomerase II, 170 kD; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase II, alpha isozyme
UniProt Protein Name
DNA topoisomerase 2-alpha
UniProt Gene Name
TOP2A
UniProt Synonym Gene Names
TOP2
UniProt Entry Name
TOP2A_HUMAN
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Product Notes
The TOP2A top2a (Catalog #AAA215960) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Recombinant Mouse Monoclonal Antibody [Clone rTOP2A/6629] reacts with Human and Mouse. and may cross-react with other species as described in the data sheet. AAA Biotech's Topoisomerase II alpha can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topoisomerase II alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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