IP (Immunoprecipitation)
(TFRC was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Hela Whole Cell Lysate2 uL anti-TFRC rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TFRC rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 84 kDaObserved band size: 100 kDa)
Rabbit anti-Human Transferrin Receptor/TFRC Monoclonal Antibody | anti-TFRC antibody
Recombinant Anti-Transferrin Receptor/TFRC Antibody, Rabbit Monoclonal
Gene Names
TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry
Purity
Protein A
Synonyms
Transferrin Receptor/TFRC, Antibody; Recombinant Anti-Transferrin Receptor/TFRC Antibody, Rabbit Monoclonal; Transferrin Receptor/TFRC/CD71 Antibody, Rabbit MAb; transferrin receptor; Anti-CD71 Antibody; Anti-p90 Antibody; Anti-T9 Antibody; Anti-TFR Antibody; Anti-TFR1 Antibody; Anti-TR Antibody; Anti-TRFR Antibody; anti-TFRC antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
016
Specificity
Human Transferrin Receptor/TFRC/CD71.
Has cross-reactivity in ELISA with Mouse TFRC
Has cross-reactivity in ELISA with Mouse TFRC
Has cross-reactivity in ELISA with Mouse TFRC
Has cross-reactivity in ELISA with Mouse TFRC
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TFRC antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human Transferrin Receptor/TFRC/CD71 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Transferrin Receptor/TFRC/CD71 (rh Transferrin Receptor/TFRC/CD71).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(TFRC was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Hela Whole Cell Lysate2 uL anti-TFRC rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TFRC rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 84 kDaObserved band size: 100 kDa)
IP (Immunoprecipitation)
(TFRC was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Hela Whole Cell Lysate2 uL anti-TFRC rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TFRC rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 84 kDaObserved band size: 100 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TFRC in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TFRC in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland.)
IHC (Immunohistochemistry)
(Immunochemical staining of human TFRC in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.)
IHC (Immunohistochemistry)
(Immunochemical staining of human TFRC in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.)
Related Product Information for anti-TFRC antibody
Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC/CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC/CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC/CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential and therapeutic target.
References
Douabin-Gicquel V., et al., 2001, Hum. Genet. 109:393-401. Ryschich, E. et al., 2004, Eur J Cancer. 40 (9):1418-22. Tosoni D., et al., 2005, Cell 123:875-888. Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
84,871 Da
NCBI Official Full Name
transferrin receptor
NCBI Official Synonym Full Names
transferrin receptor (p90, CD71)
NCBI Official Symbol
TFRC
NCBI Official Synonym Symbols
T9; TR; TFR; p90; CD71; TFR1; TRFR
NCBI Protein Information
transferrin receptor protein 1
UniProt Protein Name
Transferrin receptor protein 1
UniProt Gene Name
TFRC
UniProt Synonym Gene Names
TR; TfR; TfR1; Trfr; sTfR
UniProt Entry Name
TFR1_HUMAN
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Product Notes
The TFRC tfrc (Catalog #AAA255294) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Transferrin Receptor/TFRC Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Transferrin Receptor/TFRC can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TFRC tfrc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transferrin Receptor/TFRC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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