FCM (Flow Cytometry)
(Figure 1. Flow cytometry analysis with Anti-TSHR?M22? mAb 15 ug/mL on Expi293 cells transfected with Human TSHR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
Homo sapiens anti-Human TSHR Monoclonal Antibody | anti-TSHR antibody
Anti-TSHR (M22) mAb
Reactivity
Human
Applications
Flow Cytometry
Purity
Test:SDS-PAGE & Coomassie blue staining; Specification: Target protein band (s) >95.0%; Result: Pass
Synonyms
TSHR, Antibody; Anti-TSHR (M22) mAb; CHNG1; hTSHR-I; LGR3; anti-TSHR antibody
Host
Homo sapiens
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Test:SDS-PAGE & Coomassie blue staining; Specification: Target protein band (s) >95.0%; Result: Pass
Form/Format
Powder. Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Concentration
0.94 mg/ml (varies by lot)
Applicable Applications for anti-TSHR antibody
Flow Cytometry (FC)
Application Notes
FC: 1:100
Visual Inspection
Test:Lyophilization; Specification: Dried White powder; Result: Pass
Test:Vial Label; Specification: Label aligned and complete; Result: Pass
Test:Bottle Sealing (manual check); Specification: Sealed tight; Result: Pass
Test:Vial Label; Specification: Label aligned and complete; Result: Pass
Test:Bottle Sealing (manual check); Specification: Sealed tight; Result: Pass
Quantity
Test:Bradford method; Specification: >specified quantity; Result: Pass
Other Properties
Test:pH; Specification: 7.4 ± 0.5; Result: Pass
Reconstitution
1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5 min at room temperture to ensure the lyophilized sample settle down at the bottom of the tube
2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 106.38µL
3. After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times.
Note: Do not vortex or vigorously pipette sample
2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 106.38µL
3. After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times.
Note: Do not vortex or vigorously pipette sample
Preparation and Storage
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).
FCM (Flow Cytometry)
(Figure 1. Flow cytometry analysis with Anti-TSHR?M22? mAb 15 ug/mL on Expi293 cells transfected with Human TSHR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
FCM (Flow Cytometry)
(Figure 1. Flow cytometry analysis with Anti-TSHR?M22? mAb 15 ug/mL on Expi293 cells transfected with Human TSHR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
Product Categories/Family for anti-TSHR antibody
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The TSHR (Catalog #AAA11713) is an Antibody produced from Homo sapiens and is intended for research purposes only. The product is available for immediate purchase. The Anti-TSHR (M22) mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TSHR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). FC: 1:100. Researchers should empirically determine the suitability of the TSHR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSHR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.