Mouse Tyrosine Hydroxylase Monoclonal Antibody

Anti-Tyrosine Hydroxylase Antibody (Purified) (LNC1)

Cat. Num. AAA72838

• Reactivity: Chicken, Human, Lizard, Mouse, Non-Human Primate, Rat, Vole, Xenopus, Zebrafish
• Applications: Immunoprecipitation, Immunocytochemistry, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A chromatography
• Synonyms: Tyrosine Hydroxylase, Antibody; Anti-Tyrosine Hydroxylase Antibody (Purified) (LNC1); Tyrosine Hydroxylase (TH); anti-Tyrosine Hydroxylase antibody

• Host: Mouse
• Reactivity: Chicken, Human, Lizard, Mouse, Non-Human Primate, Rat, Vole, Xenopus, Zebrafish
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: LNC1
• Specificity: Recognizes an epitope outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: purified dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT). Identifies a single ~60 kDa band on Western Blots of HeLa cells transfected to express human tyrosine hydroxylase.
• Purity/Purification: Purified by Protein A chromatography
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)

• Applicable Applications for anti-Tyrosine Hydroxylase antibody: IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot), IHC (Immunohistochemistry)
• Antigen: Tyrosine Hydroxylase purified from PC12 cells
• Production Note: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
• Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
• Preparation and Storage: Aliquot and store at ? -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Related Product Information for anti-Tyrosine Hydroxylase antibody

Our Anti-Tyrosine Hydroxylase (TH) mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone LNC1. It detects chicken, frog, human, lizard, monkey, mouse, rat, vole, and zebrafish Tyrosine Hydroxylase (TH), and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB

TH is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine and is responsible for converting L-tyrosine to L-dopa. Synthesis of catecholamines is regulated by the interaction of TH with its’ cofactor, tetrahydrobiopterin (BH4) and the substrates L-tyrosine and molecular oxygen. In humans four TH mRNA splice variants (hTH1-hTH4) have been isolated while subprimate species rely on a single form of TH. It is known that the hTH1-hTH4 variants are identical in their catalytic domain but differ in their N-terminal regulatory domains. Importantly, LNC1 reacts with the catalytic domain of TH and thus with all four isoforms of human TH. The role of TH in the synthesis of catecholamine neurotransmitters suggests a connection between the enzyme and a number of neuropathogenic diseases characterized by irregular catecholamine levels, such as Parkinson's disease, schizophrenia, and dystonia, as well as a variety of cardiovascular diseases.

NCBI and Uniprot Product Information

• UniProt Accession #: P07101