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WB (Western Blot)
(UCHL1 Antibody western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).)
Mouse anti-Human UCHL1 Monoclonal Antibody | anti-UCHL1 antibody
UCHL1 Antibody
Gene Names
UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Synonyms
UCHL1, Antibody; UCHL1 Antibody; Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; 6---; Neuron cytoplasmic protein 95; PGP 95; PGP95; Ubiquitin thioesterase L1; UCHL1; anti-UCHL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
346CT2.2.1
Specificity
This UCHL1 monoclonal antibody is generated from mouse immunized with UCHL1 recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
223
Applicable Applications for anti-UCHL1 antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Recombinant Protein
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(UCHL1 Antibody western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).)
WB (Western Blot)
(UCHL1 Antibody western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from U266, mouse Neuro-2a, rat PC-12, C6 cell line and mouse brain tissue lysate(from left to right), using UCHL1 Antibody. AAA283430 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from U266, mouse Neuro-2a, rat PC-12, C6 cell line and mouse brain tissue lysate(from left to right), using UCHL1 Antibody. AAA283430 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IF (Immunofluorescence)
(Fluorescent image of A549 cells stained with UCHL1 Antibody. AAA283430 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of A549 cells stained with UCHL1 Antibody. AAA283430 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using UCHL1. AAA283430 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using UCHL1. AAA283430 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-UCHL1 antibody
The protein encoded by this gene belongs to the peptidase
C12 family. This enzyme is a thiol protease that hydrolyzes a
peptide bond at the C-terminal glycine of ubiquitin. This gene is
specifically expressed in the neurons and in cells of the diffuse
neuroendocrine system. Mutations in this gene may be associated
with Parkinson disease.
C12 family. This enzyme is a thiol protease that hydrolyzes a
peptide bond at the C-terminal glycine of ubiquitin. This gene is
specifically expressed in the neurons and in cells of the diffuse
neuroendocrine system. Mutations in this gene may be associated
with Parkinson disease.
Product Categories/Family for anti-UCHL1 antibody
References
Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010)
Hussain, S., et al. Leukemia 24(9):1641-1655(2010)
Ma, Y., et al. J. Cell. Biochem. 110(6):1512-1519(2010)
Wu, Y.R., et al. Clin. Chim. Acta 411 (13-14), 955-958 (2010) :
Li, L., et al. Clin. Cancer Res. 16(11):2949-2958(2010)
Hussain, S., et al. Leukemia 24(9):1641-1655(2010)
Ma, Y., et al. J. Cell. Biochem. 110(6):1512-1519(2010)
Wu, Y.R., et al. Clin. Chim. Acta 411 (13-14), 955-958 (2010) :
Li, L., et al. Clin. Cancer Res. 16(11):2949-2958(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24824
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
UniProt Entry Name
UCHL1_HUMAN
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Product Notes
The UCHL1 uchl1 (Catalog #AAA283430) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The UCHL1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UCHL1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the UCHL1 uchl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCHL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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