Mouse von Willebrand Factor/Factor VIII Related-Ag Monoclonal Antibody | anti-VWF antibody

von Willebrand Factor/Factor VIII Related-Ag (Endothelial Marker)

Cat. Num. AAA214557

• Gene Names: VWF; VWD; F8VWF
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot, Immunofluorescence
• Synonyms: von Willebrand Factor/Factor VIII Related-Ag, Antibody; von Willebrand Factor/Factor VIII Related-Ag (Endothelial Marker); Coagulation Factor VIII; Factor VIII Related Antigen; F8VWF; von Willebrand Antigen 2; von Willebrand Disease (vWD); anti-VWF antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: rVWF/1465
• Specificity: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposis sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
• Form/Format: 200ug/ml of recombinant Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 2813

• Applicable Applications for anti-VWF antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot), IF (Immunofluorescence)
• Immunogen: Recombinant fragment of human vWF protein (aa 1815-1939)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 12p13.31
• Positive Control: HUVEC cells or Tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified vWF Mouse Recombinant Monoclonal Antibody (rVWF/1465).)

WB (Western Blot)

(Western Blot Analysis A) Recombinant Protein (B) human lung lysate vWF Mouse Recombinant Monoclonal Antibody (rVWF/1465).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Mouse Recombinant Monoclonal Antibody (rVWF/1465).)

References

Germann, B.et al.2008. Pharmazie. 63: 303-307. Motta, A.et al.2009. J Biomater Sci Polym Ed. 20: 1875-1897.

NCBI and Uniprot Product Information

• NCBI GI #: 89191868
• NCBI GeneID: 7450
• NCBI Accession #: NP_000543.2
• NCBI GenBank Nucleotide #: NM_000552.4
• UniProt Accession #: P04275; Q8TCE8; Q99806
• Molecular Weight: 250kDa
• NCBI Official Full Name: von Willebrand factor preproprotein
• NCBI Official Synonym Full Names: von Willebrand factor
• NCBI Official Symbol: VWF
• NCBI Official Synonym Symbols: VWD; F8VWF
• NCBI Protein Information: von Willebrand factor
• UniProt Protein Name: von Willebrand factor
• UniProt Gene Name: VWF
• UniProt Synonym Gene Names: F8VWF; vWF