anti-Human XPD Monoclonal Antibody | anti-XPD antibody

XPD Rabbit mAb

Cat. Num. AAA314485

• Gene Names: ERCC2; EM9; TTD; XPD; COFS2; TFIIH
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity Purified
• Synonyms: XPD, Antibody; XPD Rabbit mAb; EM9; TTD; XPD; TTD1; COFS2; TFIIH; anti-XPD antibody

• Host: Recombinant Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: S08-2I3
• Purity/Purification: Affinity Purified
• Form/Format: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: 0.3mg/ml (varies by lot)

• Applicable Applications for anti-XPD antibody: IF (Immunofluorescence), WB (Western Blot)
• Immunogen: A synthetic peptide of human XPD
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.

ICC (Immunocytochemistry)

(Immunocytochemistry of XPD (green) in Hela using XPD Rabbit mAb at dilution 1/50, and DAPI(blue))

WB (Western Blot)

(Western blot detection of XPD in Jurkat,Hela cell lysates using XPD Rabbit mAb(1:500 diluted).Predicted band size:87kDa.Observed band size:80kDa.)

Related Product Information for anti-XPD antibody

ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.

Product Categories/Family for anti-XPD antibody

Primary Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 119540
• NCBI GeneID: 2068
• UniProt Accession #: P18074; Q2TB78; Q2YDY2; Q7KZU6; Q8N721
• Molecular Weight: 760
• NCBI Official Full Name: TFIIH basal transcription factor complex helicase XPD subunit
• NCBI Official Synonym Full Names: excision repair cross-complementation group 2
• NCBI Official Symbol: ERCC2
• NCBI Official Synonym Symbols: EM9; TTD; XPD; COFS2; TFIIH
• NCBI Protein Information: TFIIH basal transcription factor complex helicase XPD subunit; CXPD; BTF2 p80; TFIIH p80; TFIIH 80 kDa subunit; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; basic transcription factor 2 80 kDa subunit; xeroderma pigment
• UniProt Protein Name: TFIIH basal transcription factor complex helicase XPD subunit
• UniProt Gene Name: ERCC2
• UniProt Synonym Gene Names: XPD; XPDC; BTF2 p80; TFIIH 80 kDa subunit; TFIIH p80
• UniProt Entry Name: ERCC2_HUMAN