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WB (Western Blot)
(Detection of human YTHDF2 by western blot. Samples: Whole cell lysate (50 ug) from CCRF-CEM, MOLT-4, K-562, Jurkat, and HEL 92.1.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
YTHDF2 recombinant antibody
Rabbit anti-YTHDF2 Recombinant Monoclonal Antibody [BLR297M]
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified
Synonyms
YTHDF2, Recombinant Antibody; Rabbit anti-YTHDF2 Recombinant Monoclonal Antibody [BLR297M]; renal carcinoma antigen NY-REN-2; NY-REN-2; YTH N(6)-methyladenosine RNA binding protein 2; YTH domain-containing family protein 2; 9430020E02Rik; HGRG8; CLL-associated antigen KW-14; CAHL; YTH domain family, member 2; high-glucose-regulated protein 8; YTH N6-methyladenosine RNA binding protein 2; YTHDF2 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR297M
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for YTHDF2 recombinant antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
Between 300 and 350
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human YTHDF2 by western blot. Samples: Whole cell lysate (50 ug) from CCRF-CEM, MOLT-4, K-562, Jurkat, and HEL 92.1.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human YTHDF2 by western blot. Samples: Whole cell lysate (50 ug) from CCRF-CEM, MOLT-4, K-562, Jurkat, and HEL 92.1.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse YTHDF2 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse YTHDF2 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
FCM/FACS (Flow Cytometry)
(Detection of human YTHDF2 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human YTHDF2 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of mouse YTHDF2 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of mouse YTHDF2 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody (AAA213695) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for YTHDF2 recombinant antibody
YTHDF2 is a member of the YTH-domain containing (YT521-B homology) superfamily which also contains YTHDF1 and YTHDF3. These proteins share a conserved C-terminal YTH domain that specifically interacts with N6-methyladenosine (m6A) modified RNA. The m6A modification is present at internal sites of mRNAs and some non-coding RNAs and plays an important role in mRNA splicing, processing, and stability. YTHDF2 binding appears to promote mRNA degradation, but has minimal effect on translation efficiency. [adapted from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y5A9].
Product Categories/Family for YTHDF2 recombinant antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The YTHDF2 ythdf2 (Catalog #AAA213695) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-YTHDF2 Recombinant Monoclonal Antibody [BLR297M] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's YTHDF2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the YTHDF2 ythdf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YTHDF2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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