Application Data
(HTRF assay for YTHDF2 activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul of DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
YTHDF2 recombinant protein
Recombinant YTHDF2 protein
Synonyms
YTHDF2; N/A; Recombinant YTHDF2 protein; YTH N6-Methyladenosine RNA Binding Protein 2; HGRG8; YTHDF2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant YTHDF2 protein is supplied in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, and 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant YTHDF2 protein is suitable for use in protein-protein interaction and RNA binding assays. HTRF Assay: 3uM m6A ssDNA oligos (5'-GTTGG/ m6A/CTT-3') were incubated with YTHDF2 protein in binding buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at RT. Anti-DYKDDDDK antibody was used to detect reaction products. Binding Assay: 45 pmol biotinylated m6A ssDNA oligos (DNA sequence: 5'-GTTGCCTGTTCGTGTTGG/m6A/ CTTGCCTGT-3') were incubated with YTHDF2 protein in binding buffer including 25mM Tris-HCl pH 7.4, 150mM NaCl, 5% glycerol, 0.1% Triton X-100 for 3 hr at 4C. DNA-YTHDF2 compounds were incubated with streptavidin beads for 1 hr at 4C and pull-down products were run on a 10% SDS-PAGE gel, stained with Coomassie Blue. Biotinylated, unmethylated ssDNA oligos were used as negative control. YTHDF2 preferentially binds to ssDNA with m6A modification compared with unmodified ssDNA.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for YTHDF2 activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul of DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for YTHDF2 activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul of DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for YTHDF2 activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul of DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for YTHDF2 activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul of DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant YTHDF2 protein gel. Recombinant YTHDF2 protein was run on an 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 63.6 kDa Purity: > 90%)
SDS-PAGE
(Recombinant YTHDF2 protein gel. Recombinant YTHDF2 protein was run on an 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 63.6 kDa Purity: > 90%)
Related Product Information for YTHDF2 recombinant protein
Short Description: Recombinant YTHDF2 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_057342.2) with an N-terminal DYKDDDDK-Tag. The molecular weight of YTHDF2 is 63.6 kDa. It is suitable for use in protein-protein interaction studies and RNA binding assays.
Background: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A is also found in other RNA molecules, such as primary miRNA (pri-miRNAs). YTHDF2 (YT521-B homology domain family 2, also known as CAHL, HGRG8, NY-REN-2) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF proteins contain a highly conserved single-stranded RNA-binding domain which can specifically recognize and bind m6A-containing RNAs. An important role of YTHDF2 is in regulating mRNA stability. YTHDF2 recognizes many m6A-containing mRNAs that are not being actively translated and recruits them to cytoplasmic processing (P) bodies for degradation. YTHDF2 preserves 5'UTR methylation of stress- induced transcripts and promotes cap-independent mRNA translation by competitive binding and limiting the m6A 'eraser' FTO from demethylation.
Background: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A is also found in other RNA molecules, such as primary miRNA (pri-miRNAs). YTHDF2 (YT521-B homology domain family 2, also known as CAHL, HGRG8, NY-REN-2) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF proteins contain a highly conserved single-stranded RNA-binding domain which can specifically recognize and bind m6A-containing RNAs. An important role of YTHDF2 is in regulating mRNA stability. YTHDF2 recognizes many m6A-containing mRNAs that are not being actively translated and recruits them to cytoplasmic processing (P) bodies for degradation. YTHDF2 preserves 5'UTR methylation of stress- induced transcripts and promotes cap-independent mRNA translation by competitive binding and limiting the m6A 'eraser' FTO from demethylation.
Product Categories/Family for YTHDF2 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of YTHDF2 is 63.6kDa
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Product Notes
The YTHDF2 (Catalog #AAA59757) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "YTHDF2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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