WB (Western Blot)
(Western blot analysis of extracts of L02 cells, using YTHDF2 antibody.)
Rabbit anti-Human, Mouse YTHDF2 Polyclonal Antibody | anti-YTHDF2 antibody
YTHDF2 Antibody
Gene Names
YTHDF2; CAHL; HGRG8; NY-REN-2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
YTHDF2, Antibody; YTHDF2 Antibody; 9430020E02Rik; CAHL; CLL associated antigen KW 14; CLL-associated antigen KW-14; HGRG8; High glucose regulated protein 8; High-glucose-regulated protein 8; NY REN 2; Renal carcinoma antigen NY REN 2; Renal carcinoma antigen NY-REN-2; YTH domain family 2; YTH domain family member 2; YTH domain family protein 2; YTH N6 methyladenosine RNA binding protein 2; YTHD2_HUMAN; YTHDF 2; YTHDF2; anti-YTHDF2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
YTHDF2 antibody detects endogenous levels of YTHDF2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
529
Applicable Applications for anti-YTHDF2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human YTHDF2.
Subcellular Location
Cytosol; Nucleus
Tissue Specificity
Following heat shock stress.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of L02 cells, using YTHDF2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of L02 cells, using YTHDF2 antibody.)
IHC (Immunohiostchemistry)
(AAA323006 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323006 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from B6F10, using YTHDF2 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from B6F10, using YTHDF2 antibody. Lane 1 was treated with the blocking peptide.)
Related Product Information for anti-YTHDF2 antibody
Function: Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed:22575960, PubMed:24284625, PubMed:25412658, PubMed:25412661). Acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation (PubMed:24284625, PubMed:26046440). Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development (By similarity). Also involved in haematopoietic stem cells specification: acts by binding to m6A-containing mRNAs, leading to decrease Notch dignaling and promote endothelial to haematopoietic transition (By similarity). Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation (PubMed:26458103).
Subunit Structure: Interacts with YTHDF3 (PubMed:28106072).
Similarity: Belongs to the YTHDF2 family.
Subunit Structure: Interacts with YTHDF3 (PubMed:28106072).
Similarity: Belongs to the YTHDF2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 62 kDa
Predicted: 63 kDa
Predicted: 63 kDa
NCBI Official Full Name
YTH domain-containing family protein 2 isoform 2
NCBI Official Synonym Full Names
YTH N6-methyladenosine RNA binding protein 2
NCBI Official Symbol
YTHDF2
NCBI Official Synonym Symbols
CAHL; HGRG8; NY-REN-2
NCBI Protein Information
YTH domain-containing family protein 2
UniProt Protein Name
YTH domain-containing family protein 2
UniProt Gene Name
YTHDF2
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Product Notes
The YTHDF2 ythdf2 (Catalog #AAA323006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YTHDF2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's YTHDF2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the YTHDF2 ythdf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YTHDF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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