Mouse ZAP70 Monoclonal Antibody | anti-ZAP70 antibody

ZAP70 (Chronic Lymphocytic Leukemia Marker)

Cat. Num. AAA214834

• Gene Names: ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• Reactivity: Human. Others not known
• Applications: Immunohistochemistry, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: ZAP70, Antibody; ZAP70 (Chronic Lymphocytic Leukemia Marker); Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; Zeta chain associated protein kinase 70kDa; anti-ZAP70 antibody

• Host: Mouse
• Reactivity: Human. Others not known
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: ZAP70/2046
• Specificity: ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 312

• Applicable Applications for anti-ZAP70 antibody: IHC (Immunohistochemistry), ELISA
• Immunogen: Recombinant fragment (around aa 247-382) of human ZAP70 protein (exact sequence is proprietary)
• Positive Control: Jurkat Cells. Tonsil or lymph node.
• Cellular Localization: Cell Surface and Cytoplasmic
• Chromosome Location: 2q12
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using ZAP70 Mouse Monoclonal Antibody (ZAP70/2046).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified ZAP70 Mouse Monoclonal Antibody (ZAP70/2046). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lymph Node stained with ZAP70 Mouse Monoclonal Antibody (ZAP70/2046).)

References

Iwashima M, Irving BA, van Oers NSC, Chan AC, Weiss A. Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. Science 1994; 263:1136-9.

NCBI and Uniprot Product Information

• NCBI GI #: 31455611
• NCBI GeneID: 7535
• NCBI Accession #: NP_001070.2
• NCBI GenBank Nucleotide #: NM_001079.3
• UniProt Accession #: P43403; Q6PIA4; Q8IXD6; Q9UBS6; A6NFP4
• Molecular Weight: 70kDa
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 isoform 1
• NCBI Official Synonym Full Names: zeta chain of T cell receptor associated protein kinase 70
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK