IHC (Immunohiostchemistry)
(ZAP70 Antibody (N-term) (AAA288121)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZAP70 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit ZAP70 Polyclonal Antibody | anti-ZAP70 antibody
ZAP70 Antibody (N-term)
Gene Names
ZAP70; SRK; STD; TZK; STCD; ZAP-70
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZAP70, Antibody; ZAP70 Antibody (N-term); Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK; anti-ZAP70 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human ZAP70.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
103-130
Sequence Length
619
Applicable Applications for anti-ZAP70 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(ZAP70 Antibody (N-term) (AAA288121)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZAP70 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ZAP70 Antibody (N-term) (AAA288121)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZAP70 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ZAP70 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ZAP70 antibody detected the ZAP70 protein (arrow).)
WB (Western Blot)
(ZAP70 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ZAP70 antibody detected the ZAP70 protein (arrow).)
Related Product Information for anti-ZAP70 antibody
This gene encodes an enzyme belonging to the protein
tyrosine kinase family, and it plays a role in T-cell development
and lymphocyte activation. This enzyme, which is phosphorylated on
tyrosine residues upon T-cell antigen receptor (TCR) stimulation,
functions in the initial step of TCR-mediated signal transduction
in combination with the Src family kinases, Lck and Fyn. This
enzyme is also essential for thymocyte development. Mutations in
this gene cause selective T-cell defect, a severe combined
immunodeficiency disease characterized by a selective absence of
CD8-positive T-cells. Two transcript variants that encode different
isoforms have been found for this gene.
tyrosine kinase family, and it plays a role in T-cell development
and lymphocyte activation. This enzyme, which is phosphorylated on
tyrosine residues upon T-cell antigen receptor (TCR) stimulation,
functions in the initial step of TCR-mediated signal transduction
in combination with the Src family kinases, Lck and Fyn. This
enzyme is also essential for thymocyte development. Mutations in
this gene cause selective T-cell defect, a severe combined
immunodeficiency disease characterized by a selective absence of
CD8-positive T-cells. Two transcript variants that encode different
isoforms have been found for this gene.
Product Categories/Family for anti-ZAP70 antibody
References
Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010)
Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010) :
Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10166-10171(2010)
Kotaskova, J., et al. J Mol Diagn 12(3):328-334(2010)
Trojani, A., et al. Cancer Biomark 6(1):1-9(2010)
Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010) :
Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10166-10171(2010)
Kotaskova, J., et al. J Mol Diagn 12(3):328-334(2010)
Trojani, A., et al. Cancer Biomark 6(1):1-9(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69872
NCBI Official Full Name
tyrosine-protein kinase ZAP-70 isoform 1
NCBI Official Synonym Full Names
zeta-chain (TCR) associated protein kinase 70kDa
NCBI Official Symbol
ZAP70
NCBI Official Synonym Symbols
SRK; STD; TZK; STCD; ZAP-70
NCBI Protein Information
tyrosine-protein kinase ZAP-70
UniProt Protein Name
Tyrosine-protein kinase ZAP-70
UniProt Gene Name
ZAP70
UniProt Synonym Gene Names
SRK
UniProt Entry Name
ZAP70_HUMAN
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Product Notes
The ZAP70 zap70 (Catalog #AAA288121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 103-130. The ZAP70 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ZAP70 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ZAP70 zap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZAP70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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