WB (Western Blot)
(AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) tested by Western blot 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H4K5ac/K8ac antibody at 2 ug/mL. Lane 1: nuclear extract from untreated cells. Lane 2: nuclear extract from cells treated with sodium butyrate.)
Mouse Histone H4K5ac/K8ac Antibody | anti-H4K5ac/K8ac antibody
AbFlex Histone H4K5ac/K8ac antibody (rAb)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Wide Range Predicted
Applications
Western Blot, ELISA, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
Histone H4K5ac/K8ac; N/A; AbFlex Histone H4K5ac/K8ac antibody (rAb); anti-H4K5ac/K8ac antibody
Host
Mouse
Reactivity
Wide Range Predicted
Isotype
IgG1
Purity/Purification
Protein A Chromatography
Form/Format
140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H4K5ac/K8ac antibody
WB (Western Blot), ELISA, DB (Dot Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Antibody Type
Recombinant Antibody
Conjugation
Acetylated
Immunogen
This antibody was raised against a full-length human H4 protein containing acetyl K5 and K8 (H4K5acK8ac).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) tested by Western blot 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H4K5ac/K8ac antibody at 2 ug/mL. Lane 1: nuclear extract from untreated cells. Lane 2: nuclear extract from cells treated with sodium butyrate.)
WB (Western Blot)
(AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) tested by Western blot 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H4K5ac/K8ac antibody at 2 ug/mL. Lane 1: nuclear extract from untreated cells. Lane 2: nuclear extract from cells treated with sodium butyrate.)
ELISA
(AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) specificity tested by ELISA 10, 2 or 0.4 pmole of various biotinylated Histone H4 peptides were captured in duplicate wells of a Streptavidin coated plates. 0.5ug/mL of Histone H4K5ac/K8ac antibody was added and detected using secondary anti-mouse HRP antibody. Histone H4K5ac/K8ac antibody recognizes peptide containing both K5ac and K8ac. The presence of additional K12 and K16 acetylation does not affect detection.)
ELISA
(AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) specificity tested by ELISA 10, 2 or 0.4 pmole of various biotinylated Histone H4 peptides were captured in duplicate wells of a Streptavidin coated plates. 0.5ug/mL of Histone H4K5ac/K8ac antibody was added and detected using secondary anti-mouse HRP antibody. Histone H4K5ac/K8ac antibody recognizes peptide containing both K5ac and K8ac. The presence of additional K12 and K16 acetylation does not affect detection.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of HeLa cell chromatin and 4 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 6.7 million sequence tags were mapped to identify Histone H4K5ac/K8ac binding sites on chromosome 1.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of HeLa cell chromatin and 4 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 6.7 million sequence tags were mapped to identify Histone H4K5ac/K8ac binding sites on chromosome 1.)
Related Product Information for anti-H4K5ac/K8ac antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Histone H4K5ac/K8ac antibody (rAb) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, ELISA and Western blot; it is predicted that it will react with a wide range of sample types.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K5ac/K8ac antibody was expressed in 293 cells and contains mouse immunoglobulin heavy and light chains. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H4 molecules acetylated at Lys5 or Lys8 are distributed in overlapping, but non-identical, islands throughout the euchromatic chromosome arms.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K5ac/K8ac antibody was expressed in 293 cells and contains mouse immunoglobulin heavy and light chains. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H4 molecules acetylated at Lys5 or Lys8 are distributed in overlapping, but non-identical, islands throughout the euchromatic chromosome arms.
Product Categories/Family for anti-H4K5ac/K8ac antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,367 Da
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 4, H4
NCBI Official Symbol
HIST4H4
NCBI Official Synonym Symbols
H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
NCBI Protein Information
histone H4; histone 4, H4; OTTHUMP00000239323
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
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Product Notes
The H4K5ac/K8ac hist1h4a (Catalog #AAA60292) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Histone H4K5ac/K8ac antibody (rAb) reacts with Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4K5ac/K8ac can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, DB (Dot Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H4K5ac/K8ac hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4K5ac/K8ac, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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