DB (Dot Blot)
(Histone H4 acetyl Lys5 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Histone H4 acetyl Lys5 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide.Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide.)
Rabbit Histone H4K5ac Polyclonal Antibody | anti-H4K5ac antibody
Histone H4K5ac antibody (pAb)
DB (Dot Blot)
(Histone H4 acetyl Lys5 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Histone H4 acetyl Lys5 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide.Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide.)
DB (Dot Blot)
(Histone H4 acetyl Lys5 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Histone H4 acetyl Lys5 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide.Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide.)
WB (Western Blot)
(Histone H4 acetyl Lys5 antibody tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H4 acetyl Lys5 polyclonal antibody (1:5,000). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)
WB (Western Blot)
(Histone H4 acetyl Lys5 antibody tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H4 acetyl Lys5 polyclonal antibody (1:5,000). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)
IF (Immunofluorescence)
(Histone H4 acetyl Lys5 antibody tested by immunofluorescence. Top left: HeLa cells stained with Histone H4 acetyl Lys5 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
IF (Immunofluorescence)
(Histone H4 acetyl Lys5 antibody tested by immunofluorescence. Top left: HeLa cells stained with Histone H4 acetyl Lys5 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
ChIP (Chromatin Immunoprecipitation)
(Histone H4 acetyl Lys5 antibody tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and 50 ul of Ready-to-ChIP HeLa Chromatin per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR. Lane 1: PCR input control. Lane 2: ChIP using negative control rabbit IgG. Lane 3: ChIP using 10 ul of Histone H4 acetyl Lys5 antibody.)
ChIP (Chromatin Immunoprecipitation)
(Histone H4 acetyl Lys5 antibody tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and 50 ul of Ready-to-ChIP HeLa Chromatin per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR. Lane 1: PCR input control. Lane 2: ChIP using negative control rabbit IgG. Lane 3: ChIP using 10 ul of Histone H4 acetyl Lys5 antibody.)
ChIP (Chromatin Immunoprecipitation)
(Histone H4 acetyl Lys5 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 ug of Histone H4 acetyl Lys5 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
ChIP (Chromatin Immunoprecipitation)
(Histone H4 acetyl Lys5 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 ug of Histone H4 acetyl Lys5 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H4 molecules acetylated at Lys5 or Lys8 are distributed in overlapping, but non-identical, islands throughout the euchromatic chromosome arms.
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Product Notes
The H4K5ac hist1h4a (Catalog #AAA59886) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H4K5ac antibody (pAb) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4K5ac can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H4K5ac hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4K5ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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