Standard Curve (Sample)
Mouse CD36/SR-B3 ELISA Kit | CD36 elisa kit
Mouse CD36/SR-B3 PicoKine ELISA Kit
Gene Names
Cd36; FAT; GPIV; Scarb3
Reactivity
Mouse
Synonyms
CD36/SR-B3; N/A; Mouse CD36/SR-B3 PicoKine ELISA Kit; Platelet glycoprotein 4; CD36 molecule (thrombospondin receptor); CD36 antigen; CD36 antigen (collagen type I receptor thrombospondin receptor); CD36 molecule; CHDS7; Cluster determinant 36; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIB; GPIV; Leukocyte differentiation antigen; CD36; PAS 4 protein; PAS IV; PASIV; Platelet collagen receptor; Platelet glycoprotein IV; SCARB3; Scavenger receptor class B member 3; Thrombospondin receptor; CD36 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Cd36
Sequence Length
472
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD36 elisa kit
Background: CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.
Principle of the Assay: The Picokine™ Mouse Cd36 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Cd36 with a 96-well strip plate that is pre-coated with antibody specific for Cd36. The detection antibody is a biotinylated antibody specific for Cd36. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit includes Mouse Cd36 protein as standards. To measure Mouse Cd36, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Cd36 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Cd36 in the sample.
Principle of the Assay: The Picokine™ Mouse Cd36 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Cd36 with a 96-well strip plate that is pre-coated with antibody specific for Cd36. The detection antibody is a biotinylated antibody specific for Cd36. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit includes Mouse Cd36 protein as standards. To measure Mouse Cd36, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Cd36 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Cd36 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
52,698 Da
NCBI Official Full Name
Platelet glycoprotein 4
NCBI Official Synonym Full Names
CD36 antigen
NCBI Official Symbol
Cd36
NCBI Official Synonym Symbols
FAT; GPIV; Scarb3
NCBI Protein Information
platelet glycoprotein 4; PAS-4; GPIIIB; PAS IV; glycoprotein IIIb; fatty acid translocase; platelet glycoprotein IV
UniProt Protein Name
Platelet glycoprotein 4
UniProt Gene Name
Cd36
UniProt Synonym Gene Names
GPIIIB; GPIV
UniProt Entry Name
CD36_MOUSE
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Product Notes
The Mouse CD36 cd36 (Catalog #AAA45784) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45784 ELISA Kit recognizes Mouse CD36. It is sometimes possible for the material contained within the vial of "CD36/SR-B3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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