Standard Curve (Sample)
(Mouse CD56/NCAM1 PicoKine ELISA Kit standard curve)
Mouse CD56/NCAM1 ELISA Kit | NCAM1 elisa kit
Mouse CD56/NCAM1 PicoKine ELISA Kit
Gene Names
Ncam1; CD56; Ncam; E-NCAM; NCAM-1
Reactivity
Mouse
Synonyms
CD56/NCAM1; N/A; Mouse CD56/NCAM1 PicoKine ELISA Kit; Neural cell adhesion molecule 1; neural cell adhesion molecule 1; CD56; N-CAM-1; NCAM-1; NCAM1; NCAM1_HUMAN; NCAM1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD56
Sequence Length
1115
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
625pg/ml-40,000pg/ml
Sensitivity
< 20pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse CD56/NCAM1 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse CD56/NCAM1 PicoKine ELISA Kit standard curve)
Related Product Information for NCAM1 elisa kit
Principle of the assay: mouse CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD56 has been precoated onto 96-well plates. Standards(NSO, L20-T711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD56 amount of sample captured in plate.
Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
67,353 Da
NCBI Official Full Name
Neural cell adhesion molecule 1
NCBI Official Synonym Full Names
neural cell adhesion molecule 1
NCBI Official Symbol
Ncam1
NCBI Official Synonym Symbols
CD56; Ncam; E-NCAM; NCAM-1
NCBI Protein Information
neural cell adhesion molecule 1; N-CAM-1
UniProt Protein Name
Neural cell adhesion molecule 1
UniProt Gene Name
Ncam1
UniProt Synonym Gene Names
Ncam; N-CAM-1; NCAM-1
UniProt Entry Name
NCAM1_MOUSE
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Product Notes
The Mouse NCAM1 ncam1 (Catalog #AAA45873) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45873 ELISA Kit recognizes Mouse NCAM1. It is sometimes possible for the material contained within the vial of "CD56/NCAM1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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