WB (Western Blot)
(Western blot analysis of lysates from SW480, HT-1080 cell line and human cerebellum tissue(from left to right), using NCAM1 Antibody (C-term). AAA286993 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit NCAM1 Polyclonal Antibody | anti-NCAM1 antibody
NCAM1 Antibody (C-term)
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NCAM1, Antibody; NCAM1 Antibody (C-term); Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56; NCAM1; NCAM; anti-NCAM1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NCAM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 796-830 amino acids from the C-terminal region of human NCAM1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
848
Applicable Applications for anti-NCAM1 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Clone
RB51135
Antigen Source
HUMAN
Cellular Location
Isoform1:Cell membrane; Single-pass type I membrane protein isoform 3: Cell membrane; Lipid-anchor, GPI-anchor Isoform 5:Secreted.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from SW480, HT-1080 cell line and human cerebellum tissue(from left to right), using NCAM1 Antibody (C-term). AAA286993 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from SW480, HT-1080 cell line and human cerebellum tissue(from left to right), using NCAM1 Antibody (C-term). AAA286993 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells using NCAM1 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA286993 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells using NCAM1 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA286993 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. astroglioma section using NCAM1 Antibody (C-term). AAA286993 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. astroglioma section using NCAM1 Antibody (C-term). AAA286993 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-NCAM1 antibody
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Product Categories/Family for anti-NCAM1 antibody
References
Barton C.H.,et al.Development 104:165-173(1988).
Lanier L.L.,et al.J. Immunol. 146:4421-4426(1991).
Saito S.,et al.Lung Cancer 10:307-318(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Lanier L.L.,et al.J. Immunol. 146:4421-4426(1991).
Saito S.,et al.Lung Cancer 10:307-318(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
94574
NCBI Official Full Name
neural cell adhesion molecule 1 isoform 1
NCBI Official Synonym Full Names
neural cell adhesion molecule 1
NCBI Official Symbol
NCAM1
NCBI Official Synonym Symbols
CD56; NCAM; MSK39
NCBI Protein Information
neural cell adhesion molecule 1
UniProt Protein Name
Neural cell adhesion molecule 1
UniProt Gene Name
NCAM1
UniProt Synonym Gene Names
NCAM; N-CAM-1; NCAM-1
UniProt Entry Name
NCAM1_HUMAN
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Product Notes
The NCAM1 ncam1 (Catalog #AAA286993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCAM1 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NCAM1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NCAM1 ncam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCAM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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