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product-image-AAA150835_SC15.jpg Standard Curve (Sample)

Mouse Chemokine (C-X-C Motif) Ligand 1 (CXCL1) ELISA Kit | CXCL1 elisa kit

Mouse Chemokine (C-X-C Motif) Ligand 1 (CXCL1)(Sandwich ELISA) ELISA Kit

Gene Names
Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
Reactivity
Mouse
Applications
ELISA
Synonyms
Chemokine (C-X-C Motif) Ligand 1 (CXCL1); N/A; Mouse Chemokine (C-X-C Motif) Ligand 1 (CXCL1)(Sandwich ELISA) ELISA Kit; Mini Samples ELISA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1); CXCL1/Chemokine (C-X-C Motif) Ligand 1; NAP3; GRO1; GROa; GRO-A; MGSA-A; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein; CXCL1 elisa kit
Ordering
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of CXCL1. No significant cross-reactivity or interference between CXCL1 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
< 6.3pg/mL
Applicable Applications for CXCL1 elisa kit
ELISA
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CXCL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CXCL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

product-image-AAA150835_SC15.jpg Standard Curve (Sample)
Related Product Information for CXCL1 elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CXCL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CXCL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CXCL1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Product Categories/Family for CXCL1 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,254 Da
NCBI Official Full Name
growth-regulated alpha protein
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 1
NCBI Official Symbol
Cxcl1
NCBI Official Synonym Symbols
KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
NCBI Protein Information
growth-regulated alpha protein; C-X-C motif chemokine 1; GRO1 oncogene; KC/GR)-alpha; KC/GRO-alpha; alpha-chemokine; platelet-derived growth factor-inducible protein KC; secretory protein N51
UniProt Protein Name
Growth-regulated alpha protein
UniProt Gene Name
Cxcl1
UniProt Synonym Gene Names
Gro; Gro1; Mgsa; Scyb1; HSF
UniProt Entry Name
GROA_MOUSE

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Product Notes

The Mouse CXCL1 cxcl1 (Catalog #AAA150835) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA150835 ELISA Kit recognizes Mouse CXCL1. AAA Biotech's Chemokine (C-X-C Motif) Ligand 1 (CXCL1) can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CXCL1 cxcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chemokine (C-X-C Motif) Ligand 1 (CXCL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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