Standard Curve (Sample)
(Rat CXCL1 PicoKine ELISA Kit standard curve)
Rat CXCL1/Gro Alpha ELISA Kit | CXCL1 elisa kit
Rat CXCL1/Gro Alpha PicoKine Quick ELISA Kit
Gene Names
Cxcl1; Gro1; CINC-1
Reactivity
Rat
Applications
ELISA
Synonyms
CXCL1/Gro Alpha; N/A; Rat CXCL1/Gro Alpha PicoKine Quick ELISA Kit; CXCL1 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Cxcl1
Assay Type
Sandwich
Samples
Cell culture supernatants, serum and plasma (EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Applicable Applications for CXCL1 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Rat CXCL1 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Rat CXCL1 PicoKine ELISA Kit standard curve)
Related Product Information for CXCL1 elisa kit
Background: Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a europrotective function.
Principle of the Assay: The Quick Picokine™ Rat Cxcl1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Cxcl1 in cell culture supernatants, serum and plasma (EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Rat Cxcl1 protein as standards. To measure Rat Cxcl1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Rat Cxcl1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Cxcl1 in the sample.
Principle of the Assay: The Quick Picokine™ Rat Cxcl1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Cxcl1 in cell culture supernatants, serum and plasma (EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Rat Cxcl1 protein as standards. To measure Rat Cxcl1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Rat Cxcl1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Cxcl1 in the sample.
Product Categories/Family for CXCL1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,249 Da
NCBI Official Full Name
growth-regulated alpha protein
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 1
NCBI Official Symbol
Cxcl1
NCBI Official Synonym Symbols
Gro1; CINC-1
NCBI Protein Information
growth-regulated alpha protein; C-X-C motif chemokine 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); cytokine-induced neutrophil chemoattractant 1; platelet-derived growth factor-inducible protein KC
UniProt Protein Name
Growth-regulated alpha protein
UniProt Gene Name
Cxcl1
UniProt Synonym Gene Names
Cinc1; Gro; Scyb1; CINC-1
UniProt Entry Name
GROA_RAT
Similar Products
Product Notes
The Rat CXCL1 cxcl1 (Catalog #AAA126807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126807 ELISA Kit recognizes Rat CXCL1. AAA Biotech's CXCL1/Gro Alpha can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CXCL1 cxcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCL1/Gro Alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.